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Preclinical Study of Cell Therapy for Osteonecrosis of the Femoral Head with Allogenic Peripheral Blood-Derived Mesenchymal Stem Cells
Authors:Qiang Fu  Ning-Ning Tang  Qian Zhang  Yi Liu  Jia-Chen Peng  Ning Fang  Li-Mei Yu  Jin-Wei Liu  Tao Zhang
Affiliation:1Key Laboratory of Cell Engineering of Guizhou Province, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.;2Department of Human Anatomy, Zunyi Medical College, Zunyi, Guizhou, China.;3Department of Bone and Joint Surgery, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
Abstract:PurposeTo explore the value of transplanting peripheral blood-derived mesenchymal stem cells from allogenic rabbits (rPBMSCs) to treat osteonecrosis of the femoral head (ONFH).ResultsAfter mobilization, the cultured rPBMSCs expressed mesenchymal markers of CD90, CD44, CD29, and CD105, but failed to express CD45, CD14, and CD34. The colony forming efficiency of mobilized rPBMSCs ranged from 2.8 to 10.8 per million peripheral mononuclear cells. After local transplantation, survival of the engrafted cells reached at least 8 weeks. Therein, BMP-2 was up-regulated, while PPAR-γ mRNA was down-regulated. Additionally, bone density and bone trabeculae tended to increase gradually.ConclusionWe confirmed that local transplantation of rPBMSCs benefits ONFH treatment and that the beneficial effects are related to the up-regulation of BMP-2 expression and the down-regulation of PPAR-γ expression.
Keywords:Osteonecrosis of the femoral head (ONFH)   peripheral blood   mesenchymal stem cells (MSCs)   cell transplantation   disease model   rabbit
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