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Levels of prodynorphin mRNA in rat dentate gyrus are decreased during hippocampal kindling |
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| Authors: | B. J. Morris M. E. Moneta G. ten Bruggencate V. H llt |
| Affiliation: | 1 Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, F.R.G. 2 Physiologisches Institut, Universität München, München, F.R.G. |
| Abstract: | The effect of hippocampal kindling on the levels of prodynorphin mRNA in rat hippocampus was examined by in situ hybridization using a synthetic oligonucleotide probe. Cryostat tissue sections were hybridised with a 32P-labelled 100 mer DNA probe complementary to the coding region of rat prodynorphin mRNA, and exposed to X-ray film. In rats exhibiting stage 4 seizures, the levels of prodynorphin mRNA in the dentate gyrus were dramatically reduced compared to control animals. This suggests that the development of kindling is accompanied by a reduction in the rate of synthesis of peptides derived from prodynorphin. |
| Keywords: | Hippocampus Kindling Opioid peptide Dynorphin In situ hybridization |
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