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Creating perfused functional vascular channels using 3D bio-printing technology
Authors:Vivian K. Lee  Diana Y. Kim  Haygan Ngo  Young Lee  Lan Seo  Seung-Schik Yoo  Peter A. Vincent  Guohao Dai
Affiliation:1. Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA;2. Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA;3. Department of Bio and Brain Engineering, KAIST, Daejeon, Republic of Korea;4. Department of Biological Sciences, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA;5. Department of Radiology, Brigham and Women''s Hospital, Harvard Medical School, Boston, MA 02115, USA;6. Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA
Abstract:
We developed a methodology using 3D bio-printing technology to create a functional in vitro vascular channel with perfused open lumen using only cells and biological matrices. The fabricated vasculature has a tight, confluent endothelium lining, presenting barrier function for both plasma protein and high-molecular weight dextran molecule. The fluidic vascular channel is capable of supporting the viability of tissue up to 5 mm in distance at 5 million cells/mL density under the physiological flow condition. In static-cultured vascular channels, active angiogenic sprouting from the vessel surface was observed whereas physiological flow strongly suppressed this process. Gene expression analysis was reported in this study to show the potential of this vessel model in vascular biology research. The methods have great potential in vascularized tissue fabrication using 3D bio-printing technology as the vascular channel is simultaneously created while cells and matrix are printed around the channel in desired 3D patterns. It can also serve as a unique experimental tool for investigating fundamental mechanisms of vascular remodeling with extracellular matrix and maturation process under 3D flow condition.
Keywords:Vascular channels   3D bio-printing   Perfused vascularized tissue   Hydrogel
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