一种能与Fas结合的融合型九肽生物学活性分析

目的鉴定从噬菌体随机肽库筛选所获得的融合型多肽3A8的生物学活性。方法制备融合型多肽3A8,经纯化,采用ELISA检测融合型多肽3A8对Fas．Fc的特异性结合;细胞ELISA检测3A8能否与Jurkat细胞表面天然Fas受体结合;^3H-TdR掺入法检测3A8对Jurkat细胞增殖的影响;流式细胞仪检测细胞凋亡。结果ELISA结果显示融合型多肽能与Fas．Fc特异性结合,呈浓度剂量依赖关系。细胞ELISA实验证实3A8能与Jurkat细胞表面Fas受体结合。^3H—TdR掺入法检测发现3A8可抑制Jurkat细胞增殖,抑制率为50％。经3A8处理细胞经PI染色流式细胞仪分析,发现25．41％的Jurkat细胞发生凋亡,明显高于未经处理的对照组。结论从噬菌体随机肽库获得的融合型多肽3A8能与Jurkat细胞表面受体Fas结合,诱导细胞凋亡。

Biological characteristics of a fusion peptide 3A8 obtained from a phage display peptide library

Objective To evaluate the biological characteristics of the fusion peptide 3A8 obtained from a random phage display peptide library. Methods The binding capability of the fusion peptide 3A8 to Fas.Fc or Fas receptor on Jurkat cells was detected by ELISA and cell ELISA. The effects of the 3A8 peptide on the proliferation and apoptosis of Jurkat cells were detected by 3H-TdR uptake test and fluorescence-activated cell sorting (FACS). Results The fusion peptide 3A8 was specifically bound to Fas.Fc in a dose-dependent manner, and the binding of the 3A8 peptide to the Fas receptor on the surface of Jurkat cells was inhibited the proliferation of Jurkat cell. The apoptosis rate of the Jurkat cells incubated with the fusion peptide 3A8 for 1d was 25.41%, higher than that of normal controls. Conclusion The results show that the fusion peptide 3A8 obtained from a random phage display peptide library may bind specifically to Fas receptor on the Jurkat cell surface and inhibit Fas + cells proliferation via apoptosis.