| Institution: | (1) Department of Neurosurgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan;(2) Departments of Neurosurgery, Osaka National Hospital, Osaka, Japan;(3) Department of Physiology, School of Medicine, Keio University, Tokyo, Japan |
| Abstract: | Summary Neural stem cells (NSC) have unique differentiation-, proliferation-, and motility properties. To investigate whether they
secrete factors that interfere with the proliferation of glioma cells, we grew glioma cells in conditioned medium (CM) obtained
from cultures of neurospheres including neural stem / progenitor cells (NSPC) isolated from embryonic (E14)- or adult mouse
brain or fetal human brain. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and BrdU-labeling assays showed
that CM from NSPC (NSPC/CM) contained factor(s) that inhibited the proliferation of glioma cells by 28–87%. Filter-fractionation
of NSPC/CM revealed that the 50,000–100,000 nominal molecular weight limit (NMWL) fraction contained the inhibitory activity.
On the basis of these observations we transplanted 203G glioma cells and/or NSPC into the intrathecal space of the cisterna
magna of mice to investigate whether NSPC interfere with the proliferation of glioma cells in vivo. Mice transplanted with both 203G and NSPC survived significantly longer than did mice transplanted only with 203G. We concluded
that NSPC secrete factor(s) that may control glioma cell proliferation. |
