Induced recombination and reversion of theCDC8 gene in relation to the cell cycle of yeast |
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Authors: | H Baranowska D Zaborowska J Żuk |
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Institution: | (1) Polish Academy of Sciences, Institute of Biochemistry and Biophysics, Rakowiecka 36, PL-02-532 Warsaw, Poland |
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Abstract: | Summary The induction of mitotic recombination in theCDC8 locus was studied in a diploid strain heteroallelic forcdc8 mutations (cdc8-1/cdc8-3); mitotic reversion was studied in strainscdc8-1/cdc8-1 andcdc8-3/cdc8-3. Conversion and reversion did not occur in those cells blocked at the S stage of the cell cycle by exposure to a nonpermissive
temperature. In stationary phase cells irradiated just prior to exposure to temperature stress, the induction of recombinants
was rather low and the induction of revertants was minimal. Conversely, a significant induction ofcdc
+ occurred in logarithmic phase cells subjected to the same treatment. Irradiation of synchronously dividing cultures revealed
that intragenic recombination occurs at all three stages of the cell cycle- G1, S and G2. It was also found that UV-induced gene reversion can occur during the S and G2 stages, but not during the G1 stage of the cell cycle. |
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Keywords: | Yeast Gene conversion and mutation CDC8 locus Cell cycle |
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