耐红霉素葡萄球菌诱导克林霉素耐药的检测

目的 了解该院葡萄球菌对红霉素及克林霉素的耐药性，测定葡萄球菌对克林霉素的诱导耐药率。方法 用双纸片扩散法检测葡萄球菌对红霉素和克林霉素的耐药性，用D试验检测红霉素对克林霉素的诱导耐药率。结果 D试验阳性葡萄球菌占所检测葡萄球菌的18．2％；在葡萄球菌、金葡菌、凝固酶阴性葡萄球菌（CNS）和耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）（葡萄球菌、金葡菌、CNS和MRCNS均为红霉素耐药而克林霉素敏感）中，诱导克林霉素耐药检出率分别为63．6％、100．0％、50．0％和53．3％。结论 临床微生物实验室应加强细菌诱导克林霉素耐药的检测，以指导临床合理使用抗生素。

Detection for induction of clindamycin resistance in erythromycin-resistant isolates of Staphylococcus

Objective To investigate the resistance of Staphylococcus to erythromycin and clindamycin and to detect the percentage for inducible resistance in our hospital.Methods The susceptibilities of erythromycin and clindamycin for Staphylococcus were carried out by double disk diffusion test and the inducible resistance of erythromycin to clindamycin was detected by D-test.Results D-test positive rate was 18.2% among all Staphylococcus tested. The rate of inducible resistance to clindamycin was 63.6%, 100.0%, 50.0% and 53.3% in Staphylococcus, S. aurens,coagulase-negative Staphylococcus and methicillin-resistant coagulase-negative Staphylococcus which possessed erythromycin resistant and clindamycin sensitive by individual disk diffusion test.Conclusion The detection of inducible clindamycin resistance must be stressed in clinical microbiology laboratory to provide the reference for the clinical antimicrobial agents usage.