| Effects of cyclic tensile strain onβ-actin mRNA expression in articular chondrocytes |
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| 作者姓名： | 安丙辰 王友 汤亭亭 范启明 戴黎鸣 张晓玲 朱振安 戴尬戎 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院骨科,200011;中国科学院上海生命科学研究院上海交通大学医学院健康科学研究所;200011,上海交通大学医学院附属第九人民医院骨科;中国科学院上海生命科学研究院上海交通大学医学院健康科学研究所; |
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| 基金项目： | 上海市骨科内植物重点实验室基金上海教委重点学科建设基金上海市研发基地协作能力建设立项基金上海市重点科技支撑计划上海市卫生局青年科研项目基金上海高校选拔培养优秀青年教师科研立项基金 |
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| 摘 要： | Objective To investigate the effects of cyclic tensile strain on β- actin mRNA expression in articular chondrocytes.Methods The normal and articular chondrocytes with osteoarthritis were obtained and exposed to 3% , 6% and 15% cyclic tensile strains, respectively.The real-time quantitative PCR was used to determine the altered expressions of the P-actin mRNA under different cyclic tensile strains.Results Both 6% and 15% cyclic tensile strains significantly up-regulated the β-actin mRNA expressions (1.34±0.05, 1.36±0.04, P＜0.05) in normal articular chondrocytes, whereas only 15% cyclic tensile strain was shown to be associated with up-regulated β-actin mRNA expressions in articular chondrocytes with osteoarthritis.Conclusions The β-actin mRNA expressions of articular chondrocytes may change along with different level of cyclic tensile strains.Reaction of articular chondrocytes to cyclic tensile strains may vary under different internal or external circumstances.Therefore, the β-actin appears ineligible as a housekeeping gene in studies on biomechanical of chondrocytes.
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| 关 键 词： | 肌张力 物理刺激 软骨细胞 基因表达 RNA,信使 |
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