CD4+T细胞因子在胆道闭锁患儿肝脏组织中的表达及其意义

Objective To investigate the cytokines produced by CD4 + T cells in liver tissues of the patients with biliary atresia (BA). Methods The liver tissues of the 29 patients with BA were grounded and centrifuged. Then, the supernatants were collected. Nine patients not affected BA served as controls. The 11 cytokines produced by CD4 + T cells including IL- 12p70, IFN-γ, IL-2, IL- 10, IL-8,IL-6, IL-4, IL- 5, IL-1 β, TNF-α and TNF-β were detected using microsphere-based flow cytometry. Results Of the BA patients, the amount of IL-1β, IL-2, IL-6, IL-8, IFN-γ and TNF-α were 2 920. 69,1 106. 01,152. 22,12 614. 22,834. 18 and 161.29, respectively. The total amount of the Th1 cytokines including IL-1β, IL-2, IL-8, IL-12p70, IFN-γ, TNF-α and TNF-β was 19 504. The total amount of the proinflammatory cytokines including IL-1β, IL-2, IL-6, IL-8, IL-12p70, IFN-γ, TNFα and TNF-β was 19 653. 06. The amounts of these cytokines were significantly higher than those of the control group,which were 1 096.00,243.68,5.98,965.17,147. 28,30.56,2617.93, and 2 623.91, respectively. Conclusions The increased expressions of the cytokines expressed by the CD4 + Th1 cells may be one of the main causes of BA.

Analysis of cytokines produced by CD4+ T cells in liver tissues of the patients with biliary atresia