维拉帕米在电磁场刺激骨髓间充质干细胞增殖与分化过程中的作用

目的 研究在50Hz、0．8mT的电磁场条件下，维拉帕米（verapamil）对骨髓间充质干细胞增殖与分化的作用，以推断Ca^2＋在此过程中的作用及其内流变化情况。方法 体外培养SD大鼠骨髓间充质干细胞，取第4代细胞，应用工频电磁场及维拉帕米干预，用四甲基偶氮唑蓝比色法检测其增殖活性，按照碱性磷酸酶（ALP）检测试剂盒说明书步骤检测ALP活性，并用改良Gomori钙钻法染色定性观察。结果 50Hz、0．8mT的电磁场对骨髓间充质干细胞具有明显的促增殖（P〈0．01）、抑分化（P〈0．01）作用，但在加入维拉帕米后，这种增殖效应消失而抑分化效应减弱（P〈0．01）。结论 在50Hz、0．8mT的电磁场作用下，骨髓间充质干细胞Ca^2＋内流发生变化，且该变化在骨髓间充质干细胞向成骨方向分化过程中起部分抑制作用，而在促其增殖的过程中起主要作用。

Effects of Verapamil on the proliferation and differentiation of bone marrow mesenchymal caused by electromagnetic fields stimulation

Objective To study the effects of Verapamil on the proliferation and differentiation of bone marrow mesenchymal stimulated by electromagnetic fields(EMF) of 50 Hz and 0.8 mT. Methods The mouse bone marrow mesenchymal stem cells were isolated and cultured in vitro, the fourth-passage cells were harvested and divided into groups A,B,C and D effected by EMFs and Verapamil. The colorimetric assay was used to measure the cellular proliferation and the activity of ALP. The modified Gomori staining was also used to observe the activity of ALP. Results EMFs of 50 Hz and 0.8 mT induced a significant increase of cellular proliferation (P<0.01) and inhibited the activity of ALP (P<0.01) in comparison to the controls, but Verapamil inhibited the increase and enhanced the activity of ALP. Conclusion EMFs of 50 Hz and 0.8 mT changed intracellular free calcium ion concentration of bone marrow mesenchymal stem cells, and the change contributed to promoting the celluar proliferation and inhibiting the differentiation of bone marrow mesenchymal stem cells.