青藤碱对脑缺血再灌注损伤大鼠TXA2/PGI2的影响

目的 探讨青藤碱对大鼠脑缺血再灌注损伤后血栓素（TXA2）/前列环素（PGI2）和细胞凋亡的影响.方法 将大鼠随机分为假手术组、缺血再灌注组、Sin低剂量治疗组和青藤碱高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型.青藤碱低（30 mg/kg）、高剂量（60 mg/kg）治疗组于术前30 min分别给予大鼠腹腔注射.缺血90 min再灌注24h,进行神经功能评分,放射免疫法检测大鼠额顶部皮质TXA2和PGI2含量.结果 与缺血再灌注组比较,青藤碱高、低剂量治疗组神经功能评分较缺血再灌注组降低.青藤碱高、低剂量治疗组均能不同程度地降低缺血侧额顶部皮质TXA2的含量,减少TXA2/PGI2值,且青藤碱高剂量组明显小于青藤碱低剂量组（P均＜0.05）.结论 青藤碱对缺血再灌注脑损伤具有保护作用,其机制可能与通过降低再灌注损伤后TXA2的含量,减少TXA2/PGI2值有关,并成剂量依赖性.

Effect of Sinomenine on TXA2/PGI2 Following Cerebral Ischemic Reperfusion Injury In Rats

Objective：To study the effect of sinomenine on TXA2/PGI2 following cerebral ischemic reperfusion injury in rats.Methods：In this experiment,rats were randomly divided into 4 groups,which were sham operated group,the I/R group,the low dose of sinomenine group and the high dose of sinomenine group.The focal middle cerebral artery occlusion（MCAO） model was made by suture-occluded method.Sinomenine were given intraperitoneally to rats 30 minutes before focal cerebral ischemia operation respectively.After 90 minutes MCAO following 24 h of reperfusion,some indicators were investigated including the TXA2 and PGI2 in the frontal and parietal cortex neurological scorewith radioimmunoassay technique.Results：Compared with that of I/R group,the neurological scores were decreased obvioulsy in the low dose and the high dose of sinomenine group （all P＜0.05）.Compared with I/R group,the low dose and the high dose of sinomenine treated group dose-dependently reduced TXA2 content and TXA2/PGI2 in the frontal and parietal cortex （all P＜0.05）.Conclusion：Sinomenine may obviously reduced cerebral ischemia-reperfusion injure by decreasing dose-dependently TXA2 content and TXA2/PGI2.It plays protective effect on cerebral ischemia injury.