| 纹带棒状杆菌多位点序列分型及应用 |
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| 引用本文： | 王成玲,王嘉正,刘志国,徐帅,朱雄,李欢,王晓霞,邱小彤,魏孔娇,范仕弘,韩李超,李振军. 纹带棒状杆菌多位点序列分型及应用[J]. 中华流行病学杂志, 2021, 42(9): 1628-1634 |
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| 作者姓名： | 王成玲 王嘉正 刘志国 徐帅 朱雄 李欢 王晓霞 邱小彤 魏孔娇 范仕弘 韩李超 李振军 |
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| 作者单位： | 西藏大学医学院, 拉萨 850000;山东第一医科大学第一附属医院(山东省千佛山医院)检验医学科, 山东省医药卫生临床检验诊断学重点实验室, 济南 250000;中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京 102206;海南省三亚市人民医院检验科, 中心实验室 572000;西藏大学医学院, 拉萨 850000;中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京 102206 |
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| 基金项目： | 国家重点研发计划（2019YFC1200601，2019YFC1200705）；国家自然科学基金（82073624）；海南省自然科学基金（818QN326） |
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| 摘 要： | 目的:建立纹带棒状杆菌的多位点序列分型（MLST）方法，探讨临床分离纹带棒状杆菌的种群结构和遗传进化关系。方法:筛选出7个管家基因（ gyrA、 gyrB、 hsp65、 sodA、 secA1、 rpoB、16S rRNA），设计引物并进行PCR...
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| 关 键 词： | 纹带棒状杆菌 多位点序列分型 管家基因 序列型 |
| 收稿时间： | 2021-03-29 |
Establishment and application of a multilocus sequence typing assay for Corynebacterium striatum |
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| Wang Chengling,Wang Jiazheng,Liu Zhiguo,Xu Shuai,Zhu Xiong,Li Huan,Wang Xiaoxi,Qiu Xiaotong,Wei Kongjiao,Fan Shihong,Han Lichao,Li Zhenjun. Establishment and application of a multilocus sequence typing assay for Corynebacterium striatum[J]. Chinese Journal of Epidemiology, 2021, 42(9): 1628-1634 |
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| Authors: | Wang Chengling Wang Jiazheng Liu Zhiguo Xu Shuai Zhu Xiong Li Huan Wang Xiaoxi Qiu Xiaotong Wei Kongjiao Fan Shihong Han Lichao Li Zhenjun |
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| Affiliation: | Department of Medicine, Tibet University, Lhasa 850000, China;Department of Clinical Laboratory Medicine, The First Affiliated Hospital(Shandong Qianfoshan Hospital) of Shandong First Medical University, Shandong Medicine and Health Key Laboratory of Laboratory Medicine, Ji''nan 250000, China;State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Central and Clinical Laboratory of Sanya People''s Hospital of Hainan Province, Sanya 572000, China; Department of Medicine, Tibet University, Lhasa 850000, China;State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: | Objective To establish a multilocus sequence typing (MLST) assay for Corynebacterium (C.) striatum, explore the population structure and evolution relationship of clinical isolates of C. striatum. Methods Seven housekeeping genes (gyrA, gyrB, hsp65, sodA, secA1, rpoB, 16S rRNA) were amplified with PCR by using self-designed specific primers and sequenced. Then, the sequences were assembled with software SeqMan. The gene diversity and gene recombination characteristics were evaluated by using software DnaSP 5.10.01 and Splits tree 4.14.2. The phylogenetic tree and the minimum spanning tree were constructed based on the sequence types (ST) characteristics by using software MEGA 7.0.14 and BioNumerics, respectively. In addition, the genetic evolutionary relationship among STs were analyzed by using software eBURST 3.0. Results The expected amplification products of seven sites selected in all the test strains were obtained. Splits tree showed that the clustering of all C. striatum strains was consistent, suggesting that gene recombination is the potential driving force for the evolution of C. striatum. All of the 344 C.striatum strains were divided into 72 STs by MLST and 85.7% of the strains formed clonal complexes. CC19 was the predominant clonal complex, whereas ST16 in the clonal complex was detected in the most strains. ST had a certain geographic clustering and a certain correlation with the isolation time.Conclusions C. striatum showed high genetic diversity in China and CC19 was the predominant clonal complex. The MLST assay established in this study can be used for the typing of C. striatum, but further improvement is needed. |
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| Keywords: | Corynebacterium striatum Multilocus sequence typing Housekeeping gene Sequence type |
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