滴金免疫测定法检测不同人群布鲁氏菌病

目的探讨滴金免疫测定法在不同人群中检测布鲁氏菌感染的应用价值。方法用已建立的布病滴金免疫测定技术检测不同职业和不同感染类型人员的布病抗体,并与布病试管凝集试验作平行检测。结果滴金免疫测定法与试管凝集试验两种方法符合率为99.88%,阳性符合率达到94.25%;且本法对布病发病人群和高滴度血清样本具有更高的检测敏感性;对不同职业人群检测,接触山羊和奶牛职业人群的布鲁氏菌病抗体检出率分别为6.40%和1.32%。结论滴金免疫测定法对布病疫区人群和非疫区重点职业人员开展布病监测具有实用价值,也可用于布病的临床检测和流行病学筛查。

Detection of brucellosis among different groups of population with dot-immunogold filtration assay

The distribution of antibodies against brucella among various groups of population with different occupation and different types of Brucella infection was detected with dot-immunogold assay(DIGFA) and the results were compared with those obtained by test tube agglutination test(SAT).The results showed that the total coincidence rate between DIGFA and SAT and the coincidence on positive rate of detection were 99.88%(4003/4008) and 94.25%(82/87).respectively,and the sensitivity of DIGFA appeared to be higher in population with brucellosis and in high-titered serum samples.The detection rates of anti-brucella antibodies in individuals contacted with goat and milk cow were 6.40%(39/609)and 1.32%(45/3399) respectively.These results demonstrate that the dot-immunogold assay shows its practical value to monitor the Brucella infections among the epidemic area and individuals with different occupations in the un-epdemic area,and this method can also be applied for the clinical investigation and epidemiological survey of brucellosis.