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| 青藤碱对巨噬细胞吞噬能力的影响及机制探讨 | |
| 引用本文： | 邹同祥,张翼冠,张海港. 青藤碱对巨噬细胞吞噬能力的影响及机制探讨[J]. 中药药理与临床, 2008, 24(6) |
| 作者姓名： | 邹同祥 张翼冠 张海港 |
| 作者单位： | 1. 第三军医大学药学院新药研究中心,重庆,400038;湖南省新化县人民医院,新化417600 2. 四川省中医药科学院,成都,610041 3. 第三军医大学药学院新药研究中心,重庆,400038 |
| 摘 要： | 目的:观察青藤碱(sinomenine,SIN)对大鼠腹腔巨噬细胞吞噬能力的影响,并对其机制进行探讨.方法:大鼠随机分为对照组和青藤碱(20、60、180 mg/kg4组,酵母多糖A腹腔注射诱导急性腹膜炎,提取腹腔巨噬细胞,贴壁法纯化.鸡红细胞吞噬法测定巨噬细胞吞噬指数和吞噬率;分别采用荧光定量realtime RT-PCR和免疫组织化学方法检测TNF-α、IL-6 mRNA和蛋白表达.结果:20mg/kg青藤碱能明显提高巨噬细胞吞噬吞噬指数和吞噬率,明显降低巨噬细胞TNF-α、IL_6的表达:60、180mg/kg组能显著提高巨噬细胞吞噬指数和吞噬率,显著降低巨噬细胞TNF-α、IL-6的表达.结论:青藤碱能显著提高巨噬细胞吞噬能力,减少炎症因子表达. |
| 关 键 词： | 青藤碱 巨噬细胞 肿瘤坏死因子α 白细胞介素-6 |
Effects of sinomenine on phagocytosis ability of macrophage |
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| Zou Tongxiang,Zhang Yiguan,Zhang Haigang. Effects of sinomenine on phagocytosis ability of macrophage[J]. Pharmacology and Clinics of Chinese Materia Medica, 2008, 24(6) | |
| Authors: | Zou Tongxiang Zhang Yiguan Zhang Haigang |
| Affiliation: | Zou Tongxiang1,2,Zhang Yiguan3,Zhang Haigang2(2The People's Hospital,Xinhua County 417600,3Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,1College of Pharmacy,Third Military Medical University,Chongqing 400038) |
| Abstract: | Aim:To study the effects of sinomenine(SIN)on phagocytosis of macrophages and its potential mechanism.Method:The rats were divided into four groups randomly(n =8),i.e.one control group and 3 treated groups(SIN20,60 or 180 mg/kg,ig).Acute peritonitis was induced by zymosan A(20mg/kg)injection peritoneally in rats.Phagocytosis function of peritoneal macrophages was measured by chick-en red blood cell phagocytosis assay in vitro.mRNA and protein expression levels of interleukin 6(IL-6)and tumor necrosis factor... |
| Keywords: | sinomenine macrophage phagocytosis index phagocytosisrate interleukin 6 tumor necrosis factor alpha |
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