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Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay
Authors:M Debue  P Gautier  C Hackel  A Van Elsen  A Herzog  G Bigaignon  A Bollen
Affiliation:Service for Applied Genetics, Université Libre de Bruxelles, Nivelles, Belgium.
Abstract:
Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers, OspBpc3/pc4, outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.
Keywords:
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