甲状腺线粒体激素受体P28在甲状腺良恶性肿瘤中表达及其临床意义

目的 探讨线粒体甲状腺激素受体P28（MT-P28）基因在甲状腺乳头状癌及结节性甲状腺肿组织中的表达及其临床意义。方法 采用实时荧光定量PCR（QPCR）检测31例甲状腺乳头状癌、31例结节性甲状腺肿及其各自对应的病变旁正常组织中MT-P28 mRNA的表达水平。结果 结节性甲状腺肿组的MT-P28 mRNA相对表达量为1.57±0.14，明显高于其病变旁正常组织的0.56±0.14，差异有统计学意义（P＜0.05）。甲状腺癌组的MT-P28 mRNA相对表达量为1.73±0.30，明显高于癌旁正常组织的0.75±0.14，差异有统计学意义（P＜0.05）。MT-P28 mRNA表达在结节性甲状腺肿与其病变旁正常组织的倍比关系为2.65±0.64，差异有统计学意义（P＜0.05）；MT-P28 mRNA表达在甲状腺癌与癌旁正常组织的倍比关系为4.20±0.73，差异有统计学意义（P＜0.05）。MT-P28 mRNA表达与甲状腺癌患者性别、年龄及肿瘤大小无关（P＞0.05），而与淋巴结转移及临床分期有关（P＜0.05）。结论 MT-P28 mRNA在甲状腺良恶性肿瘤中表达水平不同，其表达水平的高低可能对甲状腺恶性病变的发生有促进作用，表达水平越高，越容易发生恶变，这对于甲状腺癌的诊断及鉴别诊断有一定的提示价值。

Clinical value of expression of thyroid hormone receptor mitochondrial P28 in benign and malignant thyroid tumor

Objective To explore the expression and clinical pathology of mitochondrial hormone receptor P28 mRNA（MT-P28 mRNA）in thyroid carcinoma tissue and nodular goiter tissue. Methods MT-P28 mRNA expression levels of 31 thyroid carcinoma tissues and 31 nodular goiter tissues were detected by quantitative PCR（QPCR）. ResultsThe mRNA level of MT-P28 in the tissue of nodular goiter patients was 1.57±0.14， higher than 0.56±0.14 of its counterparts of the lesion side tissue with statistically significant（P＜0.05）. The mRNA level of MT-P28 in the thyroid cancer patients was 1.73±0.30， higher than 0.75±0.14 of the corresponding adjacent normal tissues with statistically significant（P＜0.05）. MT-P28 mRNA level raised obviously in 31 cases of nodular goiter， and it was（2.65±0.64）times of that in the adjacent normal tissue， and the difference was statistically significant（P＜0.05）.The level of MT-P28 mRNA raised obviously in 31 cases of thyroid carcinoma compared to that of the para-tumorous tissue. The relative ratio of level of MT-P28 mRNA was 4.20±0.73 between thyroid carcinoma tissue and their para tumorous tissue， and the difference was statistically significant（P＜0.05）. MT-P28 mRNA was related with lymph node metastasis and clinical pathologicstaging（P＜0.05）， but not with gender， age and tumor size（P＞0.05）. Conclusion MT-P28 mRNA has different expression levels in benign and malignant thyroid tumors. MT-P28 mRNA levels may have a promoting role in thyroid malignant lesions. It may serve as a diagnosis and differential diagnosis value for thyroid carcinoma.