| 埃及伊蚊抗凝血因子Xa基因片段的克隆测序 |
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| 引用本文： | 朱礼华,赵彤言,刘先凯,董言德,陆宝麟.埃及伊蚊抗凝血因子Xa基因片段的克隆测序[J].中国媒介生物学及控制杂志,2001,12(6):415-417. |
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| 作者姓名： | 朱礼华 赵彤言 刘先凯 董言德 陆宝麟 |
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| 作者单位： | 军事医学科学院微生物流行病研究所全军媒介生物学重点实验室,北京, 100071 |
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| 基金项目： | 总后"九五”杰出青年基金项目资助 |
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| 摘 要： | 目的：探讨我国登革热的重要媒介蚊种埃及伊蚊唾液中的抗凝血因子Xa基因。方法：用兼并引物PCR方法从我国的埃及伊蚊基因组DNA扩增抗凝血因子Xa基因片段，克隆入测序T载体进行测序。结果：扩增出抗凝血因子Xa基因片段长度约300bp，测序后发现与报道的埃及伊蚊的cDNA基因相应序列多了一段63bp内含子，并有几个碱基和氨基酸序列变化。结论：我国海南岛的埃及伊蚊抗凝血因子Xa基因有一定差异，可能对应的是一个新的基因型。
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| 关 键 词： | 伊蚊属 埃及伊蚊 抗凝血因子Xa 聚合酶链反应 基因片断 基因克隆 测序 |
| 修稿时间： | 2001年10月22 |
Part Gene Sequence of Factor Xa-directed Anticoagulant from the Vector Mosquito
Aedes aegypti in China |
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| ZHU Li-hua,ZHAO Tong-yan,LIU Xian-kai,et al.Part Gene Sequence of Factor Xa-directed Anticoagulant from the Vector Mosquito
Aedes aegypti in China[J].Chinese Journal of Vector Biology and Control,2001,12(6):415-417. |
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| Authors: | ZHU Li-hua ZHAO Tong-yan LIU Xian-kai |
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| Abstract: | Objective: To study the factor Xagene-directed anti-coagulant (AFXA) from Aedes aegypti in China in molecular biologic levels. Methods: Degenerate oligo-nucleotide primers PCR was used to amplify from its genome DNA, then the amplified fragment was cloned into T vector to sequence. Results: A 300bp AFXA gene fragments was obtained and sequenced. There was a 63bp intron in it comparing with cDNA sequence. Conclusion: This gene fragment from Haikou strain may be a new gene type of factor Xagene-directed anti-coagulant. |
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| Keywords: | Aedes Aedes aegypti AFXA PCR |
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