COPD患者MMP-9和TIMP-1水平变化及其与IL-8和IL-2相关性研究

目的：观察慢性阻塞性肺疾病（COPD）急性加重期患者血清基质金属蛋白酶9（MMP-9）、金属蛋白酶组织抑制剂1（TIMP-1）、IL-2和IL-8含量变化。同时观察COPD患者血清MMP-9、TIMP-1与IL-2及IL-8含量的相关性。方法：选取AECOPD患者42例,健康对照者15例,采用双抗夹心ELISA法测定血清MMP-9、TIMP-1、IL-2及IL-8含量。结果：AECOPD患者血清MMP-9、TIMP-1及IL-8水平明显高于健康对照者,IL-2水平低于健康对照者,差异有统计学意义（P〈0.05）,AECOPD患者血清MMP-9、TIMP-1含量均与IL-8呈正相关,与IL-2呈负相关。结论：COPD患者血清MMP-9/TIMP-1失衡,导致肺组织降解与沉积紊乱,参与了肺气肿的发生发展。

Changes of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in serum of patients with chronic obstructive pulmonary disease and its relationship with interleukin-2 and interleukin-8

Objective： To investigate the changes of matrix metalloproteinase -9（ MMP -9） ,tissue inhibitor of metalloproteinase-1 （ TIMP-1） in the serum of patients with chronic obstructive pulmonary disease（ COPD） during acute exacerbation and its relationship with interleukin-2（ IL-2） and interleukin-8（ IL-8） . Methods： Enzyme-linked immunosorbent assay was used to measure the concentrations of MMP-9,TIMP-1,IL-2 and IL-8 in the serum of 42 patients with COPD during acute exacerbation and 15 normal subjects. Results： The concentrations of MMP-9,TIMP-1 and IL-8 in patients with COPD during acute exacerbation were significantly higher than those of the control subjects（ P 0. 05） . The concentrations of IL-2 in patients with COPD during acute exacerbation were significantly lower than that of the control subjects（ P 0. 05） . Conclusion： The serum level of MMP-9 and TIMP-1 in COPD patients is higher,suggesting MMP-9 and TIMP-1 may be involved in the pathogenesis of COPD and emphysema.