柠檬乌梅提取液预防肾草酸钙结石作用机制的研究

目的:本文旨在通过研究柠檬乌梅提取液对预防肾草酸钙结石产生的协同效应及其作用机制，为进一步研究和临床预防和治疗肾结石的应用提供理论基础。方法:将50只大鼠（体质量200～250 g）按随机数字表法分为5组，A组（对照组）、B组（成石组）、C组（柠檬提取液组）、D组（乌梅提取液组）、E组（柠檬乌梅提取液组），造模和加药处...

Study on the mechanism of lemon-dark plum extract in preventing renal calcium oxalate calculus

Objective This paper aimed to study the synergistic effect and mechanism oflemon-dark plum extract on the prevention of renal calcium oxalate calculus, so as to provide atheoretical basis for further research and clinical application in the prevention and treatment ofrenal stone. Methods A total of 50 rats (body weight 200-250 g) were divided into five groups bythe random number table method: group A (control group), group B (calculus group), group C(lemon extract group), group D (dark plum extract group), and group E (lemon-dark plum extractgroup). After modeling and dosing for 4 weeks, 24-hour urine volume and urine related indexes,blood creatinine, urea nitrogen, and other biochemical indexes were measured. The crystaldeposition and pathological changes of rat kidney were detected. The expression of osteopontin in renal tissue was detected by immunohistochemistry. Independent sample t test was used formeasurement data, and rank sum test was used for ranked data. Results The urine volume andurine creatinine in group B decreased significantly [(11.42±1.70) ml and (4.94±1.23) mmol/24 h],while the three kinds of extracts restored the urine volume and increased the urine creatininelevel [(15.58±1.61) ml, (15.70±1.69) ml, (16.43±1.55) ml; (6.21±1.17) mmol/24 h, (6.86±2.06)mmol/24 h, (7.40±1.26) mmol/24 h] (all P<0.05). Urinary calcium, uric acid, and oxalic acid inthe urine of group B increased significantly [(80.57±12.00) μmol/24 h, (2.20±0.39) mmol/24 h,and (76.98±11.89) μmol/24 h], and the lemon-dark plum extract reduced the three indexes [(35.85±5.57) μmol/24 h, (1.68±0.16) mmol/24 h, and (56.85±8.34) μmol/24 h], and its effect wassignificantly better than that of single drug extract (all P<0.05). The three kinds of extracts allreduced the levels of blood creatinine and urea nitrogen in stone rats. The renal tubular lumen of therats in group B was significantly expanded, and flake or mass calcium oxalate crystals could be seen;the three kinds of extracts significantly improved the crystal deposition and lumen expansion. Theextracts of lemon and dark plum significantly down-regulated the expression of osteopontin inducedby lithogenesis. Conclusion The lemon-dark plum extract may reduce the occurrence of renalcalculus and prevent the recurrence of renal calculus by reducing the content of blood urea nitrogenand creatinine, increasing the level of urine pH, increasing the micturition and creatinine excretionof rats, and inhibiting the formation of renal calcium oxalate crystals.