血府逐瘀汤对大鼠肝脏抗氧化酶及药物代谢酶的影响

血府逐瘀汤是一传统名方,在中国用于治疗心脑血管疾病已有100多年的历史,但血府逐瘀汤对抗氧化酶及药物代谢酶的影响尚未有报道。该研究观察血府逐瘀汤对大鼠肝脏内抗氧化酶及药物代谢酶的活性影响。采用雄性SD大鼠,血府逐瘀汤水提物3.51,7.02,14.04 g·kg-1,每日1次,连续灌胃15 d,末次给药后,动物取血,分离血清,摘除肝脏,差速离心法制备肝脏组织液、胞浆液及微粒体,测定其抗氧化酶及药物代谢酶。结果发现血府逐瘀汤对血清中AST无显著影响,高剂量组明显降低ALT的活性和Cr,BUN的含量(P<0.05);中、高剂量组显著升高GPX活性(P<0.05),高剂量显著升高CAT活性(P<0.05),对T-SOD无显著影响;在肝组织液中,血府低、中剂量组显著升高GPX活性(P<0.05);所有剂量组对GST,CAT,T-SOD的活性均无显著影响。在肝胞浆液中,低剂量组显著升高GST活性(P<0.05),低、中剂量显著升高T-SOD活性(P<0.05),对GPX,CAT无显著影响。在微粒体中,血府逐瘀汤水提物对细胞色素b5含量、NADPH-细胞色素还原酶、CYP3A,CYP2E1,UGT活性无显著影响;血府低、中剂量组可显著诱导GST活性(P<0.05);低、中、高剂量组显著升高GSH的含量。以上结果表明血府逐瘀汤可诱导GPX,CAT,T-SOD,GST和升高GSH,提示血府逐瘀汤可能具有解毒和抗氧化功能。

Effects of Xuefu Zhuyu decoction on antioxidant and drug-metabolizing enzymes in liver of rats

Xuefu Zhuyu decoction (XFZYD) is a famous traditional Chinese medicine (TCM) formula, is widely used in the treatment of cardiovascular and cerebrovascular diseases in China over one hundred years. But its effect on antioxidant and drug-metabolizing enzymes are unknown. This study was to observe the effects of Xuefu Zhuyu decoction (XFZYD) on the activities of antioxidant and drug metabolism enzymes (DMEs) in liver of rats. Male SD rats, treated with XFZYD at the dosage of 3.51, 7.02 and 14.04 g·kg^-1 per day for 15 days, serum were collected, tissue fluid, cytosols and microsomes isolated from liver tissues were prepared by centrifugation according to the standard procedure, the activities of antioxidant enzymes and drug-Metabolizing Enzymes were determined by UV-V is spectrophotometer. In serum, the activities of AST was not significantly affected by the treatment with XFZYD, at the highest dose, the levels of ALT, Cr and BUN were significantly decreased (P<0.05). GPX were significantly increased at the dose of 7.02, 14.04 g·kg^-1(P<0.05), CAT were significantly increased at the highest dose (P<0.05). T-SOD was not significantly affected by this treatment. In the liver tissue, GPX was significantly increased at the dose of 3.51, 7.02 g·kg^-1 (P<0.05), GST, CAT and T-SOD were not significantly affected following this treatment. In cytosols, GST was significantly increased at the dose of 3.51 g·kg^-1 (P<0.05), T-SOD was remarkable induced at the dose of 3.51 and 7.02 g·kg^-1 (P<0.05). In microsomes, XFZYD had no significant effect on Cytochromeb5, NADPH-Cytochrome P450 reductase, CYP3A, CYP2E1 and UGT, XFZYD significantly induced GST at the dose of 3.51 and 7.02 g·kg^-1 (P<0.05), and the level of GSH were significantly increased by XFZYD at the dose of 3.51, 7.02 and 14.04 g·kg^-1(P<0.05). These findings suggest XFZYD can induce the activities of GPX, CAT, SOD, GST and increase GSH level in liver of rats, which indicate XFZYD may have detoxification and antioxidant functions.