丹皮抗内毒素性急性肺损伤活性的谱效关系研究

分析丹皮及对照药材赤芍、白芍的高效液相特征图谱与抗内毒素性急性肺损伤活性之间的相互关系。通过HPLC-MS测定分析丹皮、赤芍、白芍各提取部位的HPLC特征图谱,用内毒素诱导大鼠急性肺损伤模型测定样品抑制蛋白和肿瘤坏死因子-α浓度的活性,运用偏最小二乘回归分析法研究谱效相关性,并用HPLC-MS/MS指认样品主要成分峰。结果显示,各样品对急性肺损伤均有一定的药效,谱效相关性研究得出4个对药效贡献率较大的成分。其中丹皮具有良好的抗急性肺损伤活性,没食子酸、芍药苷、没食子酰芍药苷和丹皮酚是其主要有效成分。

Spectrum-effect relationship of Moutan Cortex against lipopolysaccharide-induced acute lung injury

This research is to study the relationship between HPLC fingerprints of Moutan Cortex, Paeoniae Radix Rubra and Paeoniae Radix Alba and their activity on lipopolysaccharide-induced acute lung injury. HPLC fingerprints of each extract of Moutan Cortex,Paeoniae Radix Rubra and Paeoniae Radix Alba were established by an optimized HPLC-MS method. The activities of all samples against protein and tumor necrosis a factor were tested by the model of lipopolysaccharide-induced acute lung injury. The possible relationship between HPLC-MS fingerprints and the activitieswere deduced by the Partial least squares regression analysis method.Samples were analyzed by HPLC- MS/MS to identify the major peaks.The results showed that each sample had some effect on acute lung injury. Four components with a lager contribution rate of efficacy were calculated by the research of spectrum-effect relationship.Moutan Cortex exhibited good activity on acute lung injury, and gallic acid, paeoniflorin, galloylpaeoniflorin and paeonol were the main effective components.