STUDY ON THE RELATIONSHIP BETWEEN PARAOXONASE GENE POLYMORPHISM AND CORONARY ARTERIAL DISEASE IN NIDDM

Objective To ascertain the relationship between paraoxonase gene (PON) and the morbidity of coronary arterial disease (CAD) in Chinese non-insulin-dependent diabetes mellitus (NIDDM) patients. Methods The exons of PON gene were screened by polymerase chain reaction-denaturing gradient gel elec-trophoresis in 49 NIDDM patients complicated with CAD, 49 NIDDM and 101 healthy control cases of Chinese population. Results Gln-Arg191 polymorphism of the PON gene was detected in Chinese with the AIR allele frequency 0.39 and 0. 61 respectively. The genotype distribution (AA, AR and RB) of the PON gene polymor-phism was significantly different between NIDDM patients complicated with CAD and controls (NIDDM and healthy subjects). The former had a significantly higher B allele frequency (0. 79 vs 0. 62 and 0. 61, P < 0. 01). Conclusion Gln-Arg191 polymorphism of the PON gene is associated with CAD morbidity in Chinese NJDDM patients and B allele might be a risk factor.