三种肝炎病毒在肝细胞癌组织及慢性肝炎肝组织中的表达

目的:探讨乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)及庚型肝炎病毒(HGV)在肝细胞癌(HCC)及慢性肝炎肝组织中的存在状态。方法:分别采用抗HBVS,抗-HBVC,抗HCVNS5及抗-HGVNS5单克隆抗体,以免疫组织化学SPAB法检测27例HCC及37例慢性肝病患者石蜡包埋的肝组织中HBVS抗原(HBsAg)、HBVC抗原(HBcAg)、HCVNS5抗原(HCAg)和HGVNS5抗原(HGAg)。结果:HCC中检出HBsAg18例(66.7%),HCAg13例(48.2%)及HGAg9例(33.3%)。其中HBV/HCV/HGV重叠感染7例,HBV/HCV重叠感染4例,HBV单独感染7例,HCV单独感染2例,HGV单独感染2例;在慢性肝炎中检出HBsAg26例(70.3%),HCAg19例(51.4%)及HGAg12例(32.4%)。其中HBV/HCV/HGV重叠感染10例,HBV/HCV重叠感染5例,HBV单独感染11例,HCV单独感染5例,HGV单独感染2例。HBsAg、HCAg、HGAg均表达于肝细胞或肝癌细胞浆内,呈细颗粒状分布。HBcAg表达于肝细胞或肝癌细胞胞核或胞浆内。结论:慢性肝炎及HCC患者有较高的HBV、HCV及HGV感染率,HBV感染在HCC的发生过程中起重要作用,HCV和HGV的感染可能是HCC发生的相关因素。

Hepatitis Viruses in Hepatocellular Carcinoma and Chronic Hepatitis

Objective To investigate the pattern of hepatitis B virus (HBV)、hepatitis C virus (HCV) and hepatitis G virus (HGV) in tissues of hepatocellular carcinoma (HCC) and chronic hepatitis.Methods HBV S antigen、HBV C antigen、HCV NS5 antigen (HCAg) and HGV NS5 antigen (HGAg) were detected respectively in paraffin-embedded HCC tissues from 27 cases of patients with HCC and 37 cases of patient with chronic hepatitis by using immunohistochemical method (SPAB) with monoclonal anti-HBs, anti-HBc, anti-HCV NS5 and HGV NS5.Results The positive rate of HBsAg,HCAg and HGAg was 66.7%(18/27), 48.2%(13/27) and 33.3%(9/27) in HCC,respectively.There were overlapping infect of HBV/HCV/HGV in 7 cases and HBV/HCV in 4 cases.7 cases of HBV,2 cases of HCV and 2 cases of HGV were all single infection.5 cases were negative for there antigens;The positive rate of HBsAg,HCAg and HGAg was 70.3%(26/37), 51.4%(19/37) and 18.9%(7/37) in chronic hepatitis,respectively.There were overlapping infect of HBV/HCV/HGV in 10 cases and HBV/HCV in 5 cases.11 cases of HBV,5 cases of HCV and 2 cases of HGV were all single infection.HBsAg, HCAg and HGAg expressed in cytoplasma of liver or HCC cells,in thin granular pattern. HBcAg expressed in karyon or cytoplasma of liver or HCC cell.Conclusion From the study, there are high infection rates of HBV,HCV and HGV in patients with HCC or chronic hepatitis. HBV infection may play an important role, in the development of HCC while HCV and HGV infection are related factors.