|
|||||
|
|
| 大鼠体内9-硝基喜树碱代谢物的HPLC-MSn分析 | |
| 引用本文： | 张芳,王天明,闫晶超,朱恩圆,王峥涛,马越鸣,余铁流.大鼠体内9-硝基喜树碱代谢物的HPLC-MSn分析[J].中国药学杂志,2006,41(20):1586-1588. |
| 作者姓名： | 张芳 王天明 闫晶超 朱恩圆 王峥涛 马越鸣 余铁流 |
| 作者单位： | 1. 上海中医药大学中药研究所,上海,201203;上海中药标准化研究中心,上海,201203 2. 上海中医药大学中药学院,上海,201203 3. 成都切耐尔生物科技有限公司,成都,610015 |
| 摘 要： | 目的研究9-硝基喜树碱在大鼠体内的代谢物。方法静脉注射9-硝基喜树碱(6 mg·kg-1)12 h后,收集大鼠的胆汁、尿液和粪便。运用高效液相色谱和质谱联用技术检测9-硝基喜树碱的代谢产物。结果从给药后大鼠的胆汁、尿液和粪便中,发现并初步鉴定了6个主要代谢产物的化学结构。结论大鼠给予9-硝基喜树碱后,在胆汁中产生9-羟基喜树碱的葡萄糖醛酸结合物、9-乙酰胺基喜树碱、9-氨基喜树碱、9-羟基喜树碱及两个未知代谢产物;在尿液中的主要代谢产物为9-羟基喜树碱的葡萄糖醛酸结合物、9-乙酰胺基喜树碱、9-氨基喜树碱、9-羟基喜树碱;在粪便中的主要代谢产物为9-羟基喜树碱、9-乙酰胺基喜树碱及9-氨基喜树碱。 |
| 关 键 词： | 9-硝基喜树碱 代谢物 高效液相色谱-质谱 |
| 文章编号： | 1001-2494（2006）20-1586-03 |
| 收稿时间： | 2005-07-22 |
| 修稿时间： | 2005-07-22 |
Study on Metabolites of 9-Nitrocamptothecin in Rats by HPLC-MSn |
|
| ZHANG Fang,WANG Tian-ming,YAN Jing-chao,ZHU En-yuan,WANG Zheng-tao,MA Yue-ming,YU Tie-liu.Study on Metabolites of 9-Nitrocamptothecin in Rats by HPLC-MSn[J].Chinese Pharmaceutical Journal,2006,41(20):1586-1588. | |
| Authors: | ZHANG Fang WANG Tian-ming YAN Jing-chao ZHU En-yuan WANG Zheng-tao MA Yue-ming YU Tie-liu |
| Institution: | 1. Institute of Chinese Material Medica，Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China； 2. Shanghai R&D Center for Standardization of Traditional Chinese Medicine，Shanghai 201203，China； 3. College of Traditional Chinese Medicine，Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China； 4. Chengdu Qie Nai Er Bio-technology Co.，Ltd.，Chengdu 610015，China |
| Abstract: | OBJECTIVE To investigate the metabolites of 9-nitrocamptothecin in rats.METHODS After intravenous administration of 9-nitrocamptothecin(6 mg·kg-1)in rats,bile,urine and fecal excretion were collected over a 12 h period and the 9-nitrocamptathecin concentrations were determined by a HPLC-MSn method.RESULTS The major metabolites were found in rat bile,urine and fecal excretion and their chemical structures were identified primarily.CONCLUSION According to the rule of metabolism,the results indicate there are 6 metabolites in bile,4 metabolites in urine and 3 metabolites in fecal excretion. |
| Keywords: | 9-nitrocamptothecin metabolites HPLC-MS~(n) |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国药学杂志》浏览原始摘要信息 | |
| 点击此处可从《中国药学杂志》下载免费的PDF全文 | |