| 3种方法分析白蛋白空气微球的球壁蛋白 |
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| 引用本文： | 单友亮 宋青. 3种方法分析白蛋白空气微球的球壁蛋白[J]. 中国药房, 1998, 9(6): 251-252 |
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| 作者姓名： | 单友亮 宋青 |
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| 作者单位： | 第一军医大学南方医院药学部!广州市，510515，中国药品生物制品检定所血液制品室!北京市，100050，第一军医大学南方医院药学部!广州市，510515，第一军医大学南方医院药学部!广州市，510515，第一军医大学南方医院药学部心内科!广州市，510515 |
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| 摘 要： | 采用高效液相色谱（HPLC）、醋酸纤维膜电泳和半微量定氮法测定5％声振人血白蛋白空气微球制剂中球壁蛋白的含量。HPLC确证了球壁蛋白主要成分为人血白蛋白；醋酸纤维膜电泳测定空气人血白蛋白微球制剂中人血白蛋白纯度＞90％；半微量定氮法测出球壁蛋白含量为3．56％左右，99％正常值范围为2．52％～4．60％。
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| 关 键 词： | 人血白蛋白 空气白蛋白微球 球壁蛋白 |
Analysing the Shell Protein of Air-filled Albumin Microspheres by three Assay Methods |
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| SHAN Youliang,CHEN Shuyuan,CHEN Zhiliang,LIU Yili. Analysing the Shell Protein of Air-filled Albumin Microspheres by three Assay Methods[J]. China Pharmacy, 1998, 9(6): 251-252 |
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| Authors: | SHAN Youliang CHEN Shuyuan CHEN Zhiliang LIU Yili |
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| Abstract: | The shell protein of 5% air-filled microsphere preparation was detected by HPLC,acetate fibre membraneelectrophoresis and semimicro -method. Using HPLC, it was proved that the main component of shell protein was humanalbumin .Determined by acetate fibre membrane electrophoresis,it was found that the percentage purity of shell protein was morethan 90% .The content of shell protein in microsphere was detected with semimicro-quantitative nitrogen assay method and a resultof 3.56 %,was obtained and normal range is 2.52 % -4.60 %. |
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| Keywords: | human albumin air-filled albumin microsphere shell protein of microsphere |
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