兔实验性急性高眼压模型视网膜谷氨酸的变化

目的 研究兔实验性急性高眼压状态下视网膜谷氨酸含量的变化。探讨谷氨酸在青光眼高神经损害中的作用。方法 应用邻苯二甲醛衍生法、高效液相色谱仪测定视网膜谷氨酸的含量,将１８只大白兔分成３组,其中２个组１个正常对照组,每组均６只兔（６只眼）。实验１组：每只兔随机选取１只眼行生理盐水前房内高压灌注,６０ｍｍＨｇ（１ｍｍＨｇ＝０．１３３ｋＰａ）持续４ｈ。另１只眼生理盐水前房内灌注,方法同前,量压力为２０ｍｍ

Glutamate in experimental acute elevated intraocular pressure models of rabbits

Objective To measure the concentration of glutamate (Glu) in the retina of eyes with experimentally elevated intraocular pressure (IOP) Methods High performance liquid chromatography (HPLC) was utilized to measure the Glu in the retina 18 rabbits were divided into three groups, each group including 6 rabbits In one eye of each rabbit in group 1, the IOP was elevated to 60 mm Hg (1 mm Hg=0 133 kPa) by the anterior chamber perfusion with normal saline maintained for 4 hours, while the IOP of the other eye was maintained at 20 mm Hg by the same method In group 2, the same experimental method was carried out, but the elevated IOP was maintained at 30 mm Hg Group 3 was a normal control group without perfusion Results High IOP eyes (60 mm Hg and 30 mm Hg) had high glutamate concentration in the retinal tissue than that in low IOP eyes (20 mm Hg) (P<0 05) Conclusion Elevated IOP induces higher content of glutamate in the retina