纳米颗粒钛膜对成骨细胞合成功能的影响

目的通过对比不同纳米颗粒钛膜表面成骨细胞的合成能力，评价其生物相容性。方法采用直流磁控溅射法通过控制温度（常温、100 ℃、250 ℃、380 ℃）构建4级纳米颗粒钛膜，将SD乳鼠第3代成骨细胞接种于其表面及未镀膜的钛片表面，对表面成骨细胞上清液中骨钙素（OC）含量进行检测。结果在7、14 d时，随着时间的延长，各实验组和对照组样本表面的细胞上清液中OC含量均增大。在7 d时，对照组与其他各组相比，OC含量间差异均有统计学意义（P＜0.05）。在14 d时，100 ℃实验组表面OC含量增幅最大，100 ℃实验组与380 ℃实验组、对照组、空白组相比，其差异具有统计学意义（P＜0.05），250 ℃实验组与380 ℃实验组、对照组、空白组相比，其差异也具有统计学意义（P＜0.05）。结论钛表面纳米改性有利于其生物相容性，不同纳米粒径可以影响成骨细胞的合成功能。

Effect of nano-granule titanium films on synthesis of osteoblasts

Objective To compare the synthetic ability of osteoblasts on the surface of different nano-granule titanium films and investigate the correlation between nanophase titanium films and cellular biocompatibility.Methods Four different nano-granule titanium films were produced by direct current magnetron sputtering,at ambient,100 ℃,250 ℃,380 ℃ substrate temperature,respectively.Rat osteoblasts were seeded on the surface of four treated groups of titanium film samples and non-treated Ti sample(control group).The production of osteocalcin(OC)in all five groups were detected by using double antibody sandwich enzyme-linked immunosorbent assay.Results The production of OC increased gradually from day 7 to day 14 in all groups.In the control group,it showed significant differences with other five groups on day 7.On day 14,the production of OC in 100 ℃ group was the highest,and it showed significant differences with 380 ℃,control group and blank group.In 250 ℃ group,the production of OC also showed significant differences with 380℃,control group and blank group(P<0.05).Conclusion Titanium with nano-modified surface had good biocompatibility and different nano-granule titanium films could affect the synthesis of osteoblasts.