| 尘螨主要变应原Der f1融合基因真核表达载体的构建 |
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| 引用本文： | 彭江龙,崔玉宝,钱士匀,裴华,陈年根,黄幼生.尘螨主要变应原Der f1融合基因真核表达载体的构建[J].海南医学院学报,2011,17(1):4-7. |
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| 作者姓名： | 彭江龙 崔玉宝 钱士匀 裴华 陈年根 黄幼生 |
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| 作者单位： | 1. 海南医学院,海南,海口,571101
2. 盐城卫生职业技术学院,江苏,盐城,224006 |
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| 基金项目： | 国家自然科学基金资助项目(30860261)~~ |
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| 摘 要： | 目的:构建尘螨主要变应原Der f1融合基因真核表达载体,为进一步转染真核细胞表达融合蛋白奠定基础.方法:根据Genebank中Der f1基因的核酸序列(AB034946),设计引物,采用PCR法,从保存的JM109工程菌中扩增Der f1编码基因,将其连接到小鼠IL-12(mIL-12)基因3′端,组成融合基因mI...
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| 关 键 词： | 尘螨 Der f1融合基因 真核表达 |
Construction of eukaryotic expressing vector for fusion gene of Der f1, a main allergen of dermatophagoides farinae |
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| PENG Jiang-long,CUI Yu-bao,QIAN Shi-yun,PEI Hua,CHEN Nian-gen,HUANG You-sheng.Construction of eukaryotic expressing vector for fusion gene of Der f1, a main allergen of dermatophagoides farinae[J].Journal of Hainan Medical College,2011,17(1):4-7. |
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| Authors: | PENG Jiang-long CUI Yu-bao QIAN Shi-yun PEI Hua CHEN Nian-gen HUANG You-sheng |
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| Institution: | PENG Jiang-long1,CUI Yu-bao2,QIAN Shi-yun1,PEI Hua1,CHEN Nian-gen1,HUANG You-sheng1(1.Hainan Medical University,Haikou 571101,2.Yancheng Health Vocational & Technical College,Yancheng 224006,China) |
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| Abstract: | Objective:To construct eukaryotic expressing vector for fusion gene of Der f1 which is a main allergen of dermatophagoides farinae,and provide evidence for further transfection of fusion protein expressed in eukaryotic cells.Methods:The primers were designed according to nucleic acid sequences of Der f1(AB034946) from the Genebank,and Der f 1 gene from the preservative plasmid pM19-T was amplified by PCR.Then the gene was connected with murine IL-12(m IL-12) as a fusion gene mIL-12/Der f1,and cloned into th... |
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| Keywords: | Dermatophagoides farinae Der f1 fusion gene eukaryotic expression |
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