丙泊酚对大鼠单肺通气时代谢酶的影响

目的：观察丙泊酚对大鼠单肺通气时肺代谢酶的影响,为丙泊酚在胸科麻醉中的应用提供实验研究的数据.方法：40 只健康清洁级SD 大鼠随机分成四组：P 1 组：空白组（ 灌注液中无丙泊酚） ;P2 组：含36 mg/L 丙泊酚;P3 组：含24 mg/L 丙泊酚;P 4 组：含12 mg/L 丙泊酚,每组10 只.按随机顺序取大鼠进行实验,称量体重,麻醉,气管切开插管接呼吸机行单肺机械通气1 h.然后取出肺组织.-70 ℃保存,以备统一作Ca2＋ -ATPase、Na＋ -k＋ ATPase 测定.结果：肺组织Na＋-K＋ATPase 活力：P1 组通气侧活力显著低于P2、P3、P4 组通气侧（P〈0.01） ;P1 组非通气侧活力显著低于P2、P3 、P 4 组非通气侧（P〈0.01）.各组非通气侧均低于同组通气侧（P〈0.01 或0.05）.肺组织Ca2＋-ATPase 活力：P1 组通气侧显著低于P2、P3、P4 组通气侧（P〈0.01） ;P1 组非通气侧显著低于P2、P3、P4 组非通气侧（P〈0.01）.各组非通气侧均显著低于同组通气侧（P〈0.01）.结论：通过对大鼠单肺通气时的灌注实验研究发现,丙泊酚对单肺通气时的肺代谢酶有保护作用,其程度与丙泊酚浓度在一定范围内无关.

The Effect of Propofol on Metabolic Enzyme of Rats During one-lung Ventilation

Objective：To investigate the effect of propofol on metabolic enzyme of rats during one-lung ventilation.Methods：40 rats randomly divided into 4 groups（n=10, each）：group P1 （no propofol in the perfusate ）, group P2 （the concentration of propofol in perfusate was 36mg/L）, group P3（24 nag/L）, group P4 （12 rag/L）. Metabolic enzyme was quantified after one-lung ventilation .After OLV finished , lung tissue sample were harvest for lhe criterions of the Na~-K＋ATPase activity, CaE＋ATPase activity.Results：（1）The Na＋-K＋ATPase activity： as compared to the values in group P2, group P3 and group P4 ventilated lung, the Na＋-K＋ATPase activity was low in group PI ventilated lung（P〈0.01） ; as compared to the values in group P2, group P3 and group P4airproof lung, the Na＋-K＋ATPase activity was low ill group PI airproof lung（P〈0.01） ; as compared to the values in reciprocal group ventilated lung, the Na~-K＋ATPase activity was low in each group airproof lung（P〈0.01 or 0.05）.（2）The Ca2＋-ATPase activity： as compared to the values in group P2, group P3 and group P4 ventilated lung, the Ca2＋-ATPase activity was low in group Pt ventilated lung（P〈0.01） ; as compared to the values in group P2 , group P3 and group P4 airproof lung, the Ca2＋-ATPase activity was low in group Pl airproof lung（P〈0.01） ; as compared to the values in reciprocal group ventilated lung, the Ca2＋-ATPase activity was low in each group airproof lung（P〈0.01）.Conclusion：These results indicate that the propofol show the protective effects on lung metabolic enzyme of rats during one-lung ventilation , and the effects do not seem to correlate with the concentration of propofol.