沉默ERK5对胃癌SGC-7901、BGC-823细胞生物学功能的影响

目的 探讨沉默细胞外信号调节激酶5（extracellular signal regulated kinase 5，ERK5）对胃癌细胞SGC-7901、BGC-823生物学功能的影响。方法 实时荧光定量PCR法（qRT-PCR）检测人胃癌细胞株和人胃黏膜上皮细胞株ERK5 mRNA的表达水平。应用短发荚RNA（shRNA）干扰技术沉默胃癌细胞SGC-7901、BGC-823中ERK5的表达。CCK-8法检测ERK5沉默后胃癌细胞的生长能力，平板克隆实验检测细胞克隆形成能力，Transwell 实验检测细胞侵袭和迁移能力，流式细胞仪检测细胞凋亡和周期分布。结果 与胃黏膜上皮细胞GES-1相比，胃癌细胞SGC-7901、BGC-823、AGS、HGC-27中ERK5 mRNA均呈高表达，相对表达量分别为2.696±0.501、1.865±0.185、1.793±0.137和1.530±0.093（P<0.05）。ERK5-shRNA有效沉默胃癌细胞SGC-7901、BGC-823中ERK5的表达水平，沉默效率分别为（74.4±1.5）%和（69.1±3.9）%，差异有统计学意义（P<0.05）。沉默 ERK5的表达能有效抑制胃癌细胞增殖、克隆形成、迁移和侵袭能力（P<0.05），而促进胃癌细胞凋亡（P<0.05），并诱导细胞周期阻滞于G0/G1期。结论 沉默ERK5可显著抑制胃癌细胞SGC-7901、BGC-823的生长侵袭，促进细胞凋亡，ERK5可能是胃癌治疗的潜在靶点。

Effect of silencing ERK5 on the biological function of gastric cancer SGC-7901 and BGC-823 cells

Objective To investigate the effects of extracellular signal regulated kinase 5 （ERK5） on the biological functions of gastric cancer SGC-7901 and BGC-823 cells. Methods Quantitative real-time PCR（qRT-PCR） was used to detect the expression levels of ERK5 mRNA in　human gastric cancer cell lines and human gastric mucosal epithelial cell lines.The expression of ERK5 in gastric cancer cells SGC-7901 and BGC-823 was silenced by short hairpin RNA（shRNA） interference technology.After the ERK5 gene was knocked down in gastric cancer cells，cell growth and proliferation were detected by CCK-8 assay.　Cell colony formation ability　was detected by plate cloning assay，cell invasion and migration ability were detected by Transwell assay，cell apoptosis and cell cycle were detected by flow cytometry. Results Compared with GES-1 in gastric mucosal epithelial cells，ERK5 mRNA was highly expressed in gastric cancer cells SGC-7901，BGC-823，AGS，and HGC-27，the difference folds were 2.696±0.501，1.865±0.185，1.793±0.137 and 1.530±0.093，respectively（P<0.05）. ERK5-shRNA effectively silenced the expression of ERK5 in gastric cancer cells SGC-7901 and BGC-823，and the silencing efficiency were（74.4±1.5）% and （69.1±3.9）%，respectively （P<0.05）. Silencing ERK5 expression could effectively inhibit the proliferation，colony formation，migration and invasion of gastric cancer cells（P<0.05）.Silencing ERK5 expression increased gastric cancer cell apoptosis rate（P<0.05），and induced cell cycle was arrested at the G0/G1 phase. Conclusions Silencing ERK5 significantly inhibit the proliferation and invasion of gastric cancer cell lines SGC-7901 and BGC-823.ERK5 may become a potential target for the treatment of gastric cancer in the future.