3D打印胶原/羟基磷灰石支架对骨髓间充质干细胞成骨分化的作用研究

目的探讨3D打印胶原/羟基磷灰石支架对骨髓间充质干细胞成骨分化的作用。 方法分离SPF级雄性SD大鼠骨髓间充质干细胞，实验分为：对照组、浸提组、诱导组及浸提诱导组，MTT法检测对照组，浸提组的细胞增殖情况，对比分析各组细胞的碱性磷酸酶（ALP）活性，对各组细胞诱导培养17天后进行茜素红染色，观察钙结节染色情况。 结果MTT结果显示，浸提组与对照组在不同时间点的OD值比较，差异无统计学意义（P>0.05），ALP活性结果显示，不同时间点各组与对照组相比，差异均有统计学意义（P<0.05）；不同时间点浸提诱导组与浸提组相比，差异均有统计学意义（P<0.05）；诱导组在48 h及72 h与浸提组相比，差异均有统计学意义（P<0.05）。茜素红染色结果显示，对照组细胞无钙结节点，浸提组及诱导组镜下肉眼可明显观察到红色区域染色，镜下观察可见钙结节点，浸提诱导组所产生的钙结节点的数量、大小以及染色的颜色深度均明显优于其他各组。 结论3D打印胶原/羟基磷灰石支架具有生物相容性好，可促进BMSCs向成骨分化，对细胞毒性低等特点，适宜用作骨缺损的治疗。

Effect of 3D printed collagen / hydroxyapatite scaffold on osteogenic differentiation of bone marrow mesenchymal stem cells

ObjectiveTo investigate the effect of 3D printed collagen/ hydroxyapatite scaffold on osteogenic differentiation of bone marrow mesenchymal stem cells. MethodsRat BMSCs were isolated from rat bone marrow. Cell proliferation and differentiation were detected by MTT and ALP activity. After inducing cell culture for 17 days, cells in each group were stained with alizarin red to observe the staining of calcium nodules. ResultsThe MTT results showed that there was no significant difference in OD between the extraction group and the control group at different time point (P>0.05). ALP activity results showed that the extraction group had significant differences compared with the control group at different time point (P<0.05); the extraction induction group had significant differences at different time point compared with the extraction group (P<0.05); At 48 h and 72 h, the group had significant differences compared with the extraction group (P<0.05). Alizarin red staining showed that the cells in the control group had no calcium knot nodes. The red area staining was clearly observed under the microscope in the extraction group and the induction group, and the calcium knot nodes were observed under the microscope. The number, size and color depth of the knot nodes were significantly better than those of the other groups. ConclusionThe scaffold has good biocompatibility, can promote the differentiation of BMSCs to osteogenesis, and has low cytotoxicity. It is suitable for the treatment of bone defect.