牛磺酸干预对糖尿病大鼠糖脂代谢及氧化应激的影响

目的:探讨牛磺酸对2型糖尿病大鼠糖脂代谢和氧化应激的影响。方法:通过喂食SD大鼠高糖高脂饲料合并腹腔注射35 mg·kg^-1链脲佐霉素(STZ)制备2型糖尿病大鼠模型。将造模成功的大鼠随机分为模型组、二甲双胍组(400 mg·kg^-1)、牛磺酸低剂量组(400 mg·kg^-1)和牛磺酸高剂量组(600 mg·kg^-1),连续灌胃给药7周。测定各组大鼠的血糖(FBG)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL)、低密度脂蛋白胆固醇(LDL)和极低密度脂蛋白胆固醇(VLDL),血清胰岛素(FINs),并计算胰岛素抵抗系数(IRI);取肝、肾组织匀浆液,测定超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物(GPH-Px)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)含量。对氧化应激与糖脂代谢指标进行主成分分析。结果:牛磺酸高、低剂量组均具有降低血糖、TC、TG的作用,对VLD、HDL有一定影响,但无统计学差异(P>0.05)。牛磺酸高剂量组可升高肝组织GSH、T-AOC(P<0.05),降低MDA(P<0.05),也可降低肾组织MDA(P<0.05)。牛磺酸低剂量组可升高肾组织GSH,T-AOC(P<0.05)。主成分分析显示,氧化应激水平与糖脂代谢密切相关。结论:牛磺酸可改善2型糖尿病大鼠的氧化应激损伤,调节糖尿病大鼠的糖脂代谢。

Effects of taurine intervention on glucose and lipid metabolism and oxidative stress in diabetic rats

OBJECTIVE To investigate the effects of taurine on glucose and lipid metabolism and oxidative stress in type 2 diabetic rats. METHODS Rat model of type 2 diabetes were established by feeding SD rats with high-sugar and high-fat diet combined with intraperitoneal injection of 35 mg·kg^-1 streptozotocin (STZ). The rats were randomly divided into model group, metformin group (400 mg·kg^-1), low dose of taurine (400 mg·kg^-1) and high dose of taurine (600 mg·kg^-1) and intragastric administration were performed for 7 weeks. Blood glucose (FBG), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), and very low-density lipoprotein cholesterol (VLDL) and serum insulin (FINs) were measured in each group of rats, and the insulin resistance coefficient (IRI) was calculated. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxide (GPH-Px), catalase (CAT) and total antioxidant capacity (T-AOC) were measured in homogenates of liver and kidney tissues. Principal component analysis was performed on oxidative stress and glucose and lipid metabolism indicators. RESULTS Both the high and low doses of taurine had the effects of lowering blood glucose, TC and TG, and had some effects on VLD and HDL, but there was no statistical difference (P>0.05). High-dose taurine group can increase GSH and T-AOC in liver tissue (P<0.05) and decrease MDA (P<0.05). Renal tissue MDA was also reduced (P<0.05). Low-dose taurine group can increase renal tissue GSH, T-AOC (P<0.05). Principal component analysis showed that oxidative stress levels were closely related to glycolipid metabolism. CONCLUSION Taurine can improve oxidative stress injury in type 2 diabetic rats and regulate glucose and lipid metabolism in diabetic rats.