人参皂苷Rg1联合奈达铂通过Hedgehog通路抑制卵巢癌细胞OVCAR-3的增殖及机制探究

目的 探究人参皂苷Rg1联合奈达铂对卵巢癌细胞OVCAR-3增殖的影响、可能的机制以及二者的协同作用。 方法 CCK-8法检测人参皂苷Rg1、奈达铂以及两者联合对OVCAR-3细胞增殖的影响；Western blotting检测3组药物干预后Hedgehog通路关键蛋白PTCH1、SMO、Gli1表达的改变；流式细胞仪检测3组药物干预后OVCAR-3细胞周期的变化。 结果 与未进行药物干预的对照组相比，人参皂苷Rg1组、奈达铂组、联合药物组均对OVCAR-3细胞的增殖产生了抑制作用，差异有统计学意义（P < 0.05），人参皂苷Rg1与奈达铂表现出协同增效作用；与对照组相比，奈达铂组、人参皂苷Rg1组、联合药物组降低了PTCH1、SMO、Gli1蛋白的表达，差异有统计学意义（P < 0.05），人参皂苷Rg1与奈达铂表现出协同增效作用；与对照组相比，仅有联合药物组将细胞周期阻滞在G₂期（P < 0.05）。 结论 人参皂苷Rg1联合奈达铂可能通过调低Hedgehog通路相关蛋白将OVCAR-3细胞的周期阻滞在G₂期从而抑制其增殖，人参皂苷Rg1对奈达铂表现出协同增效作用。

Study on the inhibitory effects of ginsenoside Rg1 combined with nedaplatin on the proliferation of ovarian cancer cells OVCAR-3 through Hedgehog pathway and its mechanism

Objective To investigate the effects of nedaplatin combined with ginsenoside Rg1 on the proliferation of ovarian cancer cells OVCAR-3, and its possible mechanism. Methods The CCK-8 assay was used to detect the effects of ginsenoside Rg1, nedaplatin and their combination on the proliferation of OVCAR-3 cells.Western-blot was used to detect the expression levels of key proteins of Hedgehog pathway(PTCH1, SMO and Gli1).The flow cytometry was used to detect the changes of cell cycle of OVCAR-3 cells after drug intervention. Results Compared with the control group, the proliferation of OVCAR-3 cells in ginsenoside Rg1 group, nedaplatin group and combined drug group were inhibited, and the difference of which among three groups were statistically significant(P < 0.05).At the same time, ginsenoside Rg1 and nedaplatin showed synergistic effects.Compared with the control group, the expression levels of PTCH1, SMO and Gli1 proteins in nedaplatin group, ginsenoside Rg1 group and combined drug group decreased significantly, the difference of which among three groups were statistically significant(P < 0.05), and the synergistic effect between ginsenoside Rg1 and nedaplatin was found.Compared with the control group, only the combined drug group, the cell cycle was arrested at G₂ phase(P < 0.05). Conclusions Ginsenoside Rg1 combined with nedaplatin may inhibit the proliferation of OVCAR-3 cells by modulating Hedgehog pathway related proteins to arrest the cell cycle at G₂ phase, and ginsenoside Rg1 shows the synergistic effect on nedaplatin.