HBV表面抗原DNA疫苗对小鼠脏器影响的实验研究

目的：用乙型肝炎病毒（ＨＢＶ，Ｈｅｐａｔｉｔｉｓ Ｂ Ｖｉｒｕｓ）表面抗原ＤＮＡ疫苗对小鼠进行基因免疫，观察主要脏器病理形态改变，探讨该ＤＮＡ疫苗是否对肝、肾、脾等有损伤作用。方法：用乙型肝炎表面抗原（ＧＢｓＡｇ）ＤＮＡ疫苗经肌肉注射法免疫小鼠；ＥＬＩＳＡ法检测小鼠血清抗ＨＢｓＡｇ抗体水平。免疫１２周后取小鼠肝、肾、脾等组织，观察其病原形态改变情况。结果：ＤＮＡ疫苗接种１周后，免疫组小鼠血清中可检测到抗ＨＢｓＡｇ抗体，１２周后多只小鼠脾脏明显增大，肝、肾组织切片染色观察，可见免疫组小鼠的肝、肾组织均有不同程度的病理改变。结论：ＨＢｓＡｇ ＤＮＡ疫苗可诱导小鼠机体产生特异性抗体（可诱发全面、持久的免疫应答）持续１２周以上，同时伴有脾脏肿大和肝肾损伤提示，该ＤＮＡ疫苗表达产物与免疫小鼠脏器病变有一定的关系，对其临床

EFFECT OF HBsAg DNA VACCINE ON MOUSE ORGANS

To study the pathological change of organs of mice immunitied by HBsAg DNA vaccine. Methods: HBsAg vaccine was injected into muscles of mice. Anti-HBsAg antibody was analyzed by ELISA. After 12 weeks, the immunitied mice were killed and those organs were analyzed by pathological methods. Results: Anti-HBsAg antibody in mice was positive after one week. Spleens of mice were 4 folds larger than control. Kindeys and livers were severe injured. Conclusion: HBsAg vaccine can induce mice to create antibody,lasting l2 weeks and more. But,pathology change of organs indicated that the production of vaccine or related reactions may give rise to the damage of the organs of mice. This result makes us to study the safety of HBsAg vaccine further.