广州地区绝经前妇女维生素D受体基因多态性与骨密度无关

目的 研究广州地区绝经前妇女维生素D受体基因多态性的分布及其与骨密度的关系.方法 应用聚合酶链反应-限制性片段长度多态性等技术检测193例绝经前广州地区妇女维生素D受体基因型,用双能X线骨密度仪测量腰椎、股骨颈、瓦氏三角、大转子等处骨密度.结果 193例测试对象中,维生素D受体基因型分别为bb型120例(62.2%)、Bb型64例(33.2%),BB型9例(4.6%);b等位基因频率为78.76%,B等位基因频率为21.24%.基因分布符合Hardy-Weinberg定律,其基因型与所测量部位的骨密度无关.结论 未观察到绝经前妇女维生素D受体基因型与骨密度有关,维生素D受体基因BsmI多态性还不能作为预测广州地区绝经前妇女是否易患骨质疏松危险性的遗传标志物.

Vitamin D receptor gene polymorphism is not associated with bone mineral density of pre-menopausal women in Guangzhou

Objective To investigate the prevalence of vitamin D receptor(VDR)gene polymorphism in pre-menopausal women in Guangzhou and study its relationship with bone mineral density(BMD).Methods The genotypes of VDR gene in 193 per-menopausal women in Guangzhou were analyzed by polymerase chain reaction-restriction fragment length polymorphism.BMD of the lumbar spine,femoral neck,greater trochanter and Ward's triangle were measured by dual-energy X-ray absorptiometry.Results In the 193 subjects,120(66.2%)were identified as VDR bb genotype,64(33.2%)as Bb,and 9(4.6%)as BB.The b allele frequencies reached 78.76%,and B allele frequencies was 21.24%.The distribution followed the Hardy-Weinberg equilibrium.No significant difference was found in BMD among the subjects with different genotypes.Conclusion VDR genotype is not related to BMD,and VDR polymorphism can not be used as a genetic marker for predicting the risk of osteoporosis in pre-menopausal women in Guangzhou.