c-cbl 基因沉默对卵巢癌 SKOV3细胞增殖和侵袭的影响

目的：探讨 c-cbl 在卵巢癌的表达及其对卵巢癌细胞增殖、侵袭的影响。方法通过免疫组织化学法（S-P 法）检测c-cbl 蛋白在卵巢癌组织的表达；EdU 检测卵巢癌 SKOV3细胞沉默 c-cbl 基因后细胞增殖能力变化；Transwell 检测卵巢癌 SK-OV3细胞侵袭能力；Western blot 检测 P21蛋白和 P53蛋白的表达。结果 c-cbl 蛋白在卵巢癌中表达位于细胞质，在卵巢癌中， c-cbl 蛋白呈强阳性表达，在正常卵巢组织 c-cbl 蛋白呈弱阳性或者阴性表达。与正常卵巢组织比较，c-cbl 蛋白在卵巢癌表达明显增高（P＜0．05）；c-cbl 蛋白在卵巢癌的表达与 FIGO 分期相关（P＜0．05）；沉默卵巢癌 SKOV3细胞 c-cbl 基因后，卵巢癌细胞增殖能力和侵袭能力降低（P＜0．05）；沉默 c-cbl 基因后，P21和 P53蛋白表达上调（P＜0．05）。结论 c-cbl 蛋白在卵巢癌表达上调，沉默 c-cbl 基因可能与上调 P21和 P53蛋白有关。

The effect on proliferation and invasion after silence c-cbl gene in ovarian cancer SKOV3 cell

Objective To study the expression of c-cbl protein in ovarian cancer and its effect on proliferation and invasion in ovarian cancer cell .Methods The expression of c-cbl protein in ovarian tissue were detected by immunohistochemical(S-P meth-od) ,the mRNA expression of c-cbl protein in ovarian cancer SKOV3 cells were detected .the proliferation ability and migration in o-varian cancer SKOV3 cells were detected by EdU assay and Transwell assay .Western blot was used to detect the expression of P21 and P53 protein .Results c-cbl was located in the cytoplasm in ovarian cancer tissues .c-cbl showed moderate or strong positive ex-pression in ovarian cancer .In contrast ,it showed weakly positive or negative expression in normal ovarian tissue .c-cbl expressed in-creased significantly in ovarian cancer compared with those of in normal ovarian tissue(P < 0 .05) ;c-cbl expressed was associated with FIGO stage in ovarian cancer(P< 0 .05) ;silence c-cbl expression in ovarian cancer SKOV3 cells decreased the proliferation and invasion(P< 0 .05) .Silence c-cbl expression increased the expression of P21 and P53 protein(P< 0 .05) .Conclusion c-cbl was in-creased expression in ovarian cancer tissues .Silence c-cbl expression may be related to increased expression of P21 and P53 .