骨髓增生异常综合征（MDS）免疫表型研究

[目的]探讨免疫异常表型对骨髓增生异常综合征（MDS）分型诊断及鉴别诊断价值。[方法]选用多种单克隆抗体，用流式细胞测定分析仪，对79例MDS、70例良性血液病人（贫血、粒细胞减少症、血小板减少性紫癜、感染）的骨髓细胞免疫表型进行测定分析。[结果]MDS89．3％以上有二系或三系免疫袁型异常，其中CD19、CD13、CD14、CD33、CD34、HLA—DR免疫标志变化最大，明显高于正常对照组（P〈0．01）。髓系抗原表达明显增高，而且随MDS进展恶化。FAB亚型抗原表达出现规律性变化：RA→RAS→RAEB→向RAEB—T转化。较早期髓系抗原表达（如CD13、CD33）逐渐增加，而较晚期髓系抗原表达（如CD15）逐渐减少；同时伴淋系抗原表达逐渐减少；骨髓干细肜祖细胞表面抗原（如CD34、HLA—DR），随着MDS恶化发展，有逐渐明显增加异常表现，而且CD34、HLADR早期抗原表达增高者，常常预后较差，易于转化成白血病。[结论]MDS病人骨髓细胞免疫异常表型，有利于MDS诊断、分型诊断及鉴别诊断，并对治疗、预后判断有重要指导价值。

Study of immuno-phenotype of myelodysplastic syndrome

[Objective] This work is to research the immuno-phenotype significance in the classification and differentiation of Myelodysplastic Syndrome(MDS).[Methods] The immuno-phenotype of bone marrow cells was detected in 79 patients with MDS and 70 patients with benign hematological disease.[Results] More than 89.3% MDS patients had abnormality in 2 or 3 lineages of immuno-phenotype,among which CD19,13,14,33,34,HLA-DR were changed most greatly(P<0.01).Myeloid series antigens were significantly increased.They showe...