rhG-CSF对健康供者外周血和骨髓免疫特性影响的比较

本研究探讨rhG—CSF对健康供者外周血采集物（G—PB）和骨髓采集物（G—BM）免疫学特性影响的异同。用MTT法和夹心酶联免疫复合物（ELISA）法检测G—PB和G—BM中T淋巴细胞增殖能力和IL-4、IFN-7的分泌，并用流式细胞术测定两种移植物的T细胞亚群、树突状细胞（DC）亚群、单核细胞及共刺激分子CD28的表达。结果表明：G—PB中淋巴细胞，CD3^＋、CD4^＋、CD8^＋T淋巴细胞，DC1、DC2以及单核细胞的含量，CD4／CD8的比值高于G—BM（P〈0，001）。G—PB的T淋巴细胞增殖能力高于G—BM（P〈0．05）；每微升G—PB移植物中T淋巴细胞分泌细胞因子IFN-γ,IL-4的量均明显高于G—BM，且G—PB中IL-4／IFN-γ比值小于G-BM（P〈0．001），DC2与T淋巴细胞的比值也低于G—BM（P〈0．01）；而G-PB中CD4^＋、CD8^＋细胞上CD28表达的百分比和总体表达均高于G—BM（P〈0．001）。结论：rhG—CSF体内应用诱导G—PB和G—BM产生的T细胞免疫低反应性是有差异的，而两者之间的差异是G—PB和G—BM移植后移植物抗宿主病（GVHD）发生率和程度不同的免疫学基础。

Effects of rhG-CSF Mobilization on Immunological Properties of Grafts from Peripheral Blood and Bone Marrow

This study was aimed to investigate the difference of immunological properties between recombination human granulocyte colony-stimulating factor (rhG-CSF) mobilized peripheral blood grafts (G-PB) and rhG-CSF primed bone marrow grafts (G-BM). The lymphocyte proliferation ability and the quantities of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) secreted by T cells were determined by using MTT assays and sandwich ELISA; T cell subgroups, dendritic cells (DC), monocytes and the expression of CD28 costimulatory molecules on T cells were determined by multicolor flow cytometry. The results showed that the absolute numbers of lymphocytes, monocytes, CD3+, CD4+ and CD8+ T cells as well as DC1 and DC2, the ratios of CD4/CD8 in G-PB were significantly higher than those in G-BM, respectively (P < 0.001). T cell proliferation ability was significantly higher in G-PB than that in G-BM (P < 0.05). The quantities of IFN-gamma and IL-4 secreted by T cells per microliter of G-PB was significantly higher than those of G-BM, the ratios of IL-4/IFN-gamma were significantly lower in G-PB than that in G-BM (P < 0.001). As compared with G-BM, the ratio between DC2 and T-lymphocyte was significantly low in G-PB (P < 0.01), whereas the percentage and overall expression of CD28 on CD4+ and CD8+ cells were significantly high in G-PB (P < 0.05). It is concluded that T cell hyporesponsiveness of G-PB and G-BM induced by rhG-CSF in vivo were confirmed to be different, and the difference of immunological properties between G-PB and G-BM may explain the lower incidence of GVHD and lower relapse after G-BM and G-PB transplantation respectively.