银屑病易感基因位点的检测

目的：寻找中国人群银屑病的易感基因位点。方法：选取19个多态性微卫星标记,采用由荧光标记PCR、MegaBACE测序仪和与之配套的Genetic Profiler分析软件建立的荧光标记的自动基因分型体系对38个银屑病家系中的银屑病患者与亲属的外周血白细胞中提取的DNA进行 基因扫描,并用参数和非参数分析方法进行连锁分析。结果：在参数连 锁分析中,D6S1610的最大两点连锁（LOD值）为1．18（θ＝0．2）,D17S944的两大两点LOD值为1．60（θ＝0．1）;在非参数单点和多点连锁分析中,D6S1610、D17S944和D17S785的单点非参数（NPL）值均大于1．6,相应P值也小于0．05。结论：位点D6S1610、D17S944和D17785可能与中国人群银屑病相连锁。

Searching for the susceptibility loci of psoriasis in Chinese

OBJECTIVE: To search for the susceptibility genes of patients with psoriasis in Chinese. METHODS: 38 psoriasis families were determined to search for the susceptibility loci by selecting 19 reported polymorphic microsatellite DNA markers and making use of fluorescein-labeled PCR with Mega BACE sequencer. RESULTS: The significant two-point Lod score values of parametric linkage analysis were obtained with D6S1610 marker (1.18 at theta; = 0.2) and D17S944 marker (1.60 at theta; = 0.1); the NPL values greater than 1.6 (P < 0.05) of non-parametric analysis were obtained with D6S1610 marker, D17S944 marker and D17S785 marker. CONCLUSION: A putative linkage to D6S1610 marker, D17S944 marker and D17S785 marker.