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人M5型白血病-SCID小鼠模型的建立及白血病病理变化的分析
引用本文:周斌,居颂光,居颂文,谢芳,张学光. 人M5型白血病-SCID小鼠模型的建立及白血病病理变化的分析[J]. 细胞与分子免疫学杂志, 2007, 23(6): 501-503
作者姓名:周斌  居颂光  居颂文  谢芳  张学光
作者单位:苏州大学第一附属医院临床免疫实验室,苏州大学生物技术研究所,江苏,苏州,215007
基金项目:国家自然科学基金;江苏省卫生厅"135工程"医学重点人才基金
摘    要:目的:建立人M5型白血病细胞系SHI-1/SCID(重症联合免疫缺陷)小鼠模型,探讨白血病细胞的接种密度与SCID小鼠成瘤率及病理变化的关系。方法:将不同密度的人白血病细胞SHI-1注射至SCID小鼠腹腔。定期尾静脉取血并以流式细胞术(FCM)监测肿瘤细胞表面标志CD14及CD137L的变化。通过病理组织学检查和FCM分析SCID小鼠的骨髓、肝脏、脾脏和肾脏中白血病细胞的浸润及病理变化。结果:将不同密度的SHI-1细胞移植至小鼠腹腔,均可发现瘤块生长。同时中、高剂量组小鼠的主要组织器官呈现不同程度的肿瘤细胞的浸润。最早在移植3周后即可在尾静脉检测到肿瘤细胞,并与注射细胞量相关。结论:建立的SHI-1/SCID小鼠模型为人M5型白血病的研究提供了有价值的动物模型。

关 键 词:白血病,急性  SCID小鼠  模型
文章编号:1007-8738(2007)06-0501-03
修稿时间:2006-05-15

Establishment of human acute monocytic leukemia model in severe combined immunodeficient (SCID) mice and the analysis of pathological changes
ZHOU Bin,JU Song-guang,JU Song-wen,XIE Fang,ZHANG Xue-guang. Establishment of human acute monocytic leukemia model in severe combined immunodeficient (SCID) mice and the analysis of pathological changes[J]. Chinese journal of cellular and molecular immunology, 2007, 23(6): 501-503
Authors:ZHOU Bin  JU Song-guang  JU Song-wen  XIE Fang  ZHANG Xue-guang
Affiliation:Jiangsu Provincial Key Lab of Clinical Immunology, Biotechnological Institute, Soochow University, Soochow 215007, China
Abstract:AIM: To establish a model of human M5 leukemia and investigate the pathomorphological change in severe combined immunodeficient (SCID) mice after being transplanted with SHI-1 cells. METHODS: Various dose of SHI-1 cells were transplanted i.p. into SCID mice. Serial tail vein samples at regular intervals were prepared for detecting the expression of CD14, CD137L on SHI-1 cells by flow cytometry (FCM). All dying mice were dissectted and the liver, spleen, kidney and bone marrow were examined to detect the appearance and distribution of the SHI-1 cells by FCM and/or immunohistochemistry. RESULTS: Tumors appeared in the abdomenion of the mice treated with various dose of SHI-1 cells. However, the dissemination of SHI-1 cells in murine tissues was found only in the groups of middle/high dose. SHI-1 cells were firstly found in tail vein samples 3 weeks after transplantation, and the time of engraftment was related to the dose of cells. CONCLUSION: The SHI-1/SCID mice was constructed, which provided a useful tool to investigate acute monocytic leukemia(AML).
Keywords:leukemia  acute  SCID mice  model
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