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| 脱细胞猪角膜的制备及其生物学性能观察 | |
| 引用本文： | 郭梁,刘源,张勇杰,赵宇. 脱细胞猪角膜的制备及其生物学性能观察[J]. 山东医药, 2010, 50(42): 4-5 |
| 作者姓名： | 郭梁 刘源 张勇杰 赵宇 |
| 作者单位： | 山东大学齐鲁医院,济南,250012;中国人民解放军第四军医大学组织工程中心 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的制备脱细胞猪角膜（DPC）并观察其生物学性能。方法用高渗盐溶液浸泡、低浓度酶消化、冻干的方法制备DPC。对比观察DPC、人羊膜（HAM）、兔角膜（RC）的光学特性和机械强度,阿贝折射仪测量折射率,PE分光光度计测量透射率,小型英斯特朗仪测量抗拉强度和弹性模量。结果折射率DPC为1.331 3、HAM为1.332 2、RC为1.334 0;透射率DPC为70%、HAM为72%、RC为92%;抗拉强度DPC为2.4 mPa、HAM为3.6mPa、RC为4.5 mPa;弹性模量DPC为3.8 mPa、HAM为0.78 mPa、RC为5.3 mPa。三种材料的折射率相比,P〉0.05;DPC与HAM的透射率相比,P〉0.05;三种材料的抗拉强度和弹性模量相比,P均〈0.05。结论成功制备了DPC,DPC具备与HAM相似的光学特性,DPC有其自身独特的力学特性,能耐受手术缝合。 |
| 关 键 词： | 组织工程角膜 脱细胞猪角膜 人羊膜 兔角膜 折射率 透射率 抗拉强度 弹性模量 |
Preparation and optical and mechanical properties measurements of DPC |
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| GUO Liang,LIU Yuan,ZHANG Yong-jie,ZHAO Yu. Preparation and optical and mechanical properties measurements of DPC[J]. Shandong Medical Journal, 2010, 50(42): 4-5 | |
| Authors: | GUO Liang LIU Yuan ZHANG Yong-jie ZHAO Yu |
| Affiliation: | GUO Liang1,LIU Yuan,ZHANG Yong-jie,ZHAO Yu(1 Qilu Hospital,Shandong University,Jinan 250012,P.R.China) |
| Abstract: | Objective To prepare the decellularized porcine cornea(DPC) and compare the optical and mechanical properties of DPC.Methods DPC was prepared by decellularized and freeze-dried process.Refractive indices(RI) of DPC,human amnion(HAM) and rabbit cornea(RC) were recorded using a Abbe refractometer.Transmittances of the three materials were measured on a UV/vis spectrometer.The tensile strength and elastic modulus of DPC,HAM and RC were determined on an Instron micro tester.Results The RI of DPC was 1.331 3,and... |
| Keywords: | tissue engineering cornea decellularized porcine cornea human amnion rabbit cornea refractive index transmittance tensile strength elastic modulus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |