PHA对CIK细胞增殖和细胞毒作用影响的研究

为了探讨植物血凝素 (PHA)对细胞因子诱导的杀伤细胞 (CIK)增殖和细胞毒作用的影响 ,分离健康人外周血单个核细胞 ,用含自体血浆的培养液培养CIK细胞和PHA先刺激 2 4小时的PHA CIK细胞。用51 Cr释放法检测两种细胞对K5 6 2细胞、食管癌细胞和初治急性白血病病人骨髓幼稚细胞的细胞毒作用。结果表明 :两种细胞体外增殖能力较强 ,且PHA CIK细胞强于CIK细胞 (P <0 .0 5 )。两种细胞对不同靶细胞均有较强的杀伤能力。在效靶比为 2 0∶1、10∶1时 ,PHA CIK细胞对K5 6 2细胞和初治急性白血病病人骨髓幼稚细胞的杀伤作用强于CIK细胞 (P <0 .0 5 )。结论 :PHA增加CIK细胞的增殖能力和抗肿瘤活性 ,可作为生物治疗应用于临床。

Effect of Phytohemagglutinin on Proliferation and Cytoxicity of Cytokine-Induced Killer Cells

The purpose was to investigate the effect of phytohemagglutinin (PHA) on proliferation and cytotoxicity of cytokine-induced killer (CIK). Peripheral blood mononuclear cells (PBMNCs) from healthy donors were divided into two groups. Cells were resuspended and maintained in complete medium containing of 10% autologous plasma. CIK cells were cultured by traditional method in group one. The other group cells were added PHA to stimulate PBMNCs for 24 hours, then cultured like incubating CIK cells. Their cytotoxicity to different target cells was evaluated by 51Cr release assay. The results showed that the proliferation multiples of CIK and PHA-CIK cells were both high, however, the latter was much higher than CIK with significance (P< 0.05). Cells in each group cells showed high cytotoxicity. At the same high effector/target ratio PHA-CIK cells cytotoxicity was stronger than CIK cells when targets were K562 cells or acute leukemia cells (P<0.05). In conclusion, PHA-CIK cells exhibt stronger proliferation and cytotoxicity than CIK cells, and the result provides an experimental basis for biotherapy.