急性髓系白血病患者骨髓溶血磷脂酰胆碱酰基转移酶1的表达分析

目的: 探讨急性髓系白血病（acute myeloid leukemia, AML）患者骨髓中溶血磷脂酰胆碱酰基转移酶1（lysophosphatidylcholine acyltransferase 1, LPCAT1）的表达和临床意义。方法: 应用实时定量PCR技术检测60例不同临床分型的AML患者和27例健康对照骨髓标本LPCAT1 mRNA的表达。结果: 与对照组相比,AML患者LPCAT1 mRNA的表达水平显著降低（P<0.001）。60例AML患者中27例（45％）存在LPCAT1 mRNA低表达,而27例对照均未见LPCAT1 mRNA低表达改变,差异有统计学意义（χ2=17.618,P<0.001）。LPCAT1 mRNA低表达与年龄、性别、白细胞计数、血小板计数、血红蛋白、FAB分型及核型危险分级等均无临床相关性（P>0.05）。LPCAT1 mRNA区分AML患者的受试者工作特征（ROC）曲线下面积为0.896（95% CI:0.825~0.967,P<0.001）,LPCAT1 mRNA表达的截断值为0.173时,敏感性和特异性分别为88.3%和81.5%。 结论: LPCAT1低表达是AML中的常见分子事件,或可用于AML辅助诊断。

Expression analysis of lysophosphatidylcholine acyltransferase 1 gene in patients with acute myeloid leukemia

Objective:To explore the expression status of lysophosphatidylcholine acyltransferase 1 (LPCAT1) gene and its clinical significance in patients with acute myeloid leukemia (AML). Methods:Quantitative real time PCR was used to examine the LPCAT1 mRNA expression in bone marrow samples from 60 primary AML patients and 27 healthy donors. Results: LPCAT1 mRNA expression was significant lower in AML than controls(P<0.001). LPCAT1 mRNA low expression was found in 27 (45%) of 60 AML cases but none in the 27 controls, the difference was statistically significant (χ2=17.618,P<0.001).There was no correlation between the low expression of LPCAT1 mRNA with the age, sex, white blood cell count, platelet count, hemoglobin concentration, FAB subtypes and AML karyotypic groups (P>0.05). The receiver operating characteristic (ROC) curve revealed LPCAT1 mRNA expression might serve as a potential diagnostic marker for differentiating AML from controls with an area under the ROC curve of 0.896 (95% CI:0.825~0.967, P<0.001). At the cut off value of 0.173 of LPCAT1 mRNA expression, the sensitivity and the specificity were 88.3% and 81.5%, respectively.Conclusion: LPCAT1 low expression is a frequent molecular event in AML, and it may be used to assist the diagnosis of AML.