| Selection of Immunogenic Peptide Mimics of Male Worm Origin of Schistosoma Japonicum using Phage Display Peptide Library |
| |
| 作者姓名： | 陈欲晓 易新元 曾宪芳 王林纤 唐连飞 章洁 McreynoldsLarry |
| |
| 作者单位： | Department of Immunology，Xiangya School of Medicine，Central South University，Department of Parasitology，Xiangya School of Medicine，Central South University，Department of Parasitology，Xiangya School of Medicine，Central South University，Department of Parasitology，Xiangya School of Medicine，Central South University，Department of Parasitology，Xiangya School of Medicine，Central South University，Department of Parasitology，Xiangya School of Medicine，Central South University，Molecular Parasitology Group，New England Biolabs，Inc. Changsha 410078，China，Changsha 410078，China，Changsha 410078，China，Changsha 410078，China，Changsha 410078，China，Changsha 410078，China，Beverly，MA01915，USA |
| |
| 基金项目： | ThisprojectwassupportedbyWHO TDR(No.980268) |
| |
| 摘 要： | Phagepeptidedisplaytechniquehasbecomeaverypopu larmethodofstudyinawidevarietyofresearchworkson accountofitsversatility,simplicityandcosteffectiveness. Ithasbeenwidelyusedforidentificationofproteinsand hasprovidedaconvenientmethodologytoobtainligands fors…
|
| 关 键 词： | 噬菌体 展示技术 筛选方法 日本血吸虫 雄虫源性 免疫学 模拟短肽 免疫原 |
Selection of Immunogenic Peptide Mimics of Male Worm Origin of Schistosoma Japonicum using Phage Display Peptide Library |
| |
| Yuxiao CHEN Xinyuan YI Xianfang ZENG Linqian WANG,Lianfei TANG Jie ZHANG and Mcreynolds Larry.Selection of Immunogenic Peptide Mimics of Male Worm Origin of Schistosoma Japonicum using Phage Display Peptide Library[J].Chinese Journal of Microbiology and Immunology,2004,2(1):66-69. |
| |
| Authors: | Yuxiao CHEN Xinyuan YI Xianfang ZENG Linqian WANG Lianfei TANG Jie ZHANG and Mcreynolds Larry |
| |
| Institution: | [1]DepartmentofImmunology,XiangyaSchoolofMedicine,CentralSouthUniversity,Changsha410078,China [3]DepartmentofParasitology,XiangyaSchoolofMedicine,CentralSouthUniversity,Changsha410078,China [4]MolecularParasitologyGroup,NewEnglandBiolabs,Inc.,Beverly,MA01915,USA |
| |
| Abstract: | To select the immunogenic short peptide mimics of male worm origin of Schistosoma japonicum (Sj) and to explore their protection effect against schistosomiasis in mice, the random phage display peptide library of 12-mer was screened with IgG to soluble male worm antigen of Sj, and the specific positive clones selected through three rounds of screenings were detected by Dot-ELISA, and then injected subcutaneously into mice for vaccination and protection assessment against Sj. It was found that 18 randomly picked phage displayed clones all showed definite antigenicity with various intensities. The pooled phages displayed clones could induce production of specific antibodies and cause 31.72% of worm reduction rate and 51.54% of egg reduction rate in mice, revealing a significant difference (P<0.001) in comparison with those of the controls. It concludes that the short peptide mimics of male worm origin of Sj obtained by affinity screening phage display peptide library can elicit partial protection against this pathogen. |
| |
| Keywords: | Schistosoma japonicum Male worm Short peptide mimic Phage peptide library Screening |
| 本文献已被 CNKI 维普 等数据库收录！ |
