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| 人胚嗅球嗅鞘细胞原代培养的实验研究 | |
| 引用本文： | 任玉水,闫军浩,王洪美,陈琳,黄红云,周长满,姜晓荣,田国忠. 人胚嗅球嗅鞘细胞原代培养的实验研究[J]. 解剖科学进展, 2010, 16(2): 97-100 |
| 作者姓名： | 任玉水 闫军浩 王洪美 陈琳 黄红云 周长满 姜晓荣 田国忠 |
| 作者单位： | 1. 北京市虹天济神经科学研究院;北京康复中心神经外科,北京,100144 2. 北京大学医学部解剖与组织胚胎学系,北京,100191 3. 北京市虹天济神经科学研究院;北京大学医学部解剖与组织胚胎学系,北京,100191 4. 北京市虹天济神经科学研究院 |
| 基金项目： | 国家自然科学基金资助项目(No30971527) |
| 摘 要： | 目的拟建立人胚胎嗅球嗅鞘细胞的培养方法并探讨这些细胞在活性、纯度方面是否具备临床应用水平。方法用含有促进细胞生长的谷氨酰胺、转铁蛋白、生物素、硒酸纳的无血清纯化液纯化嗅鞘细胞,胰蛋白酶消化收集细胞,制成单细胞悬液。采用台盼蓝染色法进行活性测定,P75NGFR和S-100双标免疫荧光染色鉴定,以Hoechst复染鉴定OECs的纯度。结果可见比较典型的嗅鞘细胞:双极或梭形、多突起形和扁圆形,主要以梭形和多突起形细胞为主。细胞活性大于95%,纯度大于90%。结论实验建立的人胚胎嗅球嗅鞘细胞原代培养的方法可行,细胞活性大于95%,纯度和均一性已达到临床应用水平。 |
| 关 键 词： | 嗅鞘细胞 细胞培养 纯化 免疫荧光 人胚 |
The primary culture for human embryonic olfactory ensheathing cells of olfactory bulb |
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| REN Yu-shui,YAN Jun-hao,WANG Hong-mei,CHEN Lin,HUANG Hong-yun,ZHOU Chang-man,JIANG Xiao-rong,TIAN Guo-zhong. The primary culture for human embryonic olfactory ensheathing cells of olfactory bulb[J]. Progress of Anatomical Sciences, 2010, 16(2): 97-100 | |
| Authors: | REN Yu-shui YAN Jun-hao WANG Hong-mei CHEN Lin HUANG Hong-yun ZHOU Chang-man JIANG Xiao-rong TIAN Guo-zhong |
| Affiliation: | 1.Beijing Hongtianji Neuroscience Academy;2.Department of Neurosurgery;Beijing Rehabilitation Center;Beijing 100144;3.Department of Anatomy & Embryo;Peking University Health Science Center;Beijing 100191 China |
| Abstract: | Objective To establish a cultural method for human embryonic olfactory ensheathing cells(OECs)of olfactory bulb in order to obtain cells with better viability and purification to satisfy the clinical application.Methods The olfactory ensheathing cells were purified with serum-free purification liquid containing glutamine,transferrin,biotin,sodium selenate,and were digested with trypsinase and collected as the single cell suspension.The cell viability was determined with trypan blue staining,the purity of ol... |
| Keywords: | olfactory ensheathing cells cell culture purification immunofluorescence human embryo |
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