双葛解酲丸对大鼠酒精性脂肪肝的保护作用

目的:观察双葛解酲丸对酒精性脂肪肝模型大鼠血清酶活力及肝脏指数的影响,探讨本处方对酒精性脂肪肝的保护作用.方法:以56度红星二锅头酒ig(10 mL·kg-1,2次/d,连续8周)的方法建立酒精性脂肪肝大鼠模型.第9周将造模成功大鼠分为模型对照组、海王对照组(海王金樽片1.5 g·kg-1)、双葛解酲丸(双葛)高、中、低剂量组(按生药量计为20,10,5 g·kg-1 ·d-1),连续给药4周,同期设正常对照组.给药结束后,禁食12h,股动脉取血检测血清γ-谷氨酰转移酶(γ-GT)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活力及肝脏指数.结果:模型组γ-GT,ALT,AST活力及肝脏指数较正常对照组显著性升高(P ＜0.05,P＜0.01),体质量显著性降低(P＜0.01)；双葛高、中、低剂量组、海王组γ-GT,ALT,AST活力及肝脏指数较模型组显著性降低(P ＜0.05,P＜0.01),体质量显著性上升(P＜0.01)；双葛高剂量组γ-GT,ALT,AST活力较中低剂量组显著性降低(P＜0.05,P＜0.01).结论:双葛解酲丸能降低γ-GT,ALT,AST活力,减轻脂肪在肝脏的蓄积,降低肝细胞损害,保护肝脏功能,对酒精性脂肪肝确有疗效.

Protective Effects of Shuangge Jiecheng Pill on Alcoholic Fatty Liver in Rats

Objective:To observe the protective effects of Shuangge Jiecheng Pill on ferments alcoholic fatty liver in rats.Method:The rat alcoholic fatty liver model was induced by 56%Hongxing wine by intragastric administration with 10 mL·kg-1,twice per day for 8 weeks.Then,the successfully modeled rats were successively administrated for 4 weeks,and randomly divided into model group,HW group(Haiwang Jinzun tablet,1.5 g·kg-1)and Shuangge Jiecheng pill(SG) group with high dose(HD),middle dose(MD) and low dose(LD)(crude drug 20,10,5 g·kg-1·d-1),and the control group was set up in the same period.After administration and fasting for 12 h later,the blood was obtained from arteria cruralis for detecting theactivity of γ-glutamy(transferaseγ-GT),alanine aminotransferase(ALT) and aspartate aminotrans-ferase(AST),and the liver index was observed also.Result:Compared with the control group,avtivity of γ-glutamyltransferase(γ-GT),alanine aminotransferase(ALT) and aspartate aminotransferase(AST) and the liver indexes in the model group were obviously increased(P<0.05) while the body weight was decreased(P<0.01).Compared with the model group,activity of γ-GT,ALT and AST and the liver indexes in the HW and SG group of HD,MD and LD were obviously decreased(P<0.05,P<0.01) while the body weight was increased(P<0.01),and the indexes in SG group of HD were obviously decreased(P<0.05,P<0.01) than those of SG group of MD and LD.Conclusion:Shuangge Jiecheng Pill has an protective actions on for alcoholic fatty liver.