| 高灵敏度吸光光度法测定生物样品中微量硒 |
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| 引用本文： | 熊永革,黄胜堂. 高灵敏度吸光光度法测定生物样品中微量硒[J]. 微量元素与健康研究, 2002, 19(2): 62-63 |
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| 作者姓名： | 熊永革 黄胜堂 |
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| 作者单位： | 咸宁医学院检验教研室,湖北,咸宁,437100 |
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| 摘 要： | 研究了在吐温 -80存在下 ,Se( )与碘化物和罗丹明 B形成三元离子缔合物分光光度测定硒的方法。缔合物在 5 80 nm处有强吸收 ,硒在 0～ 3 .0 μg/2 5 ml范围内符合比尔定律。表观摩尔吸光系数为 4.66× 1 0 5L·mol- 1· cm- 1。试用于人体血清及头发样品中微量硒测定 ,结果满意。
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| 关 键 词： | 硒 碘化物 罗丹明B 分光光度法 |
| 文章编号： | 1005-5320(2002)02-0062-02 |
| 修稿时间： | 2002-03-03 |
High Sensitive Spectrophotometric Determination of trace selenium |
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| XIONG Yong-Ge,HUANG Sheng-Tang. High Sensitive Spectrophotometric Determination of trace selenium[J]. Studies of Trace Elements and Health, 2002, 19(2): 62-63 |
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| Authors: | XIONG Yong-Ge HUANG Sheng-Tang |
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| Abstract: | In this paper an ion-associate complex of selenium was formed with iodide and Rhodamine B in the presence of tween-80.Maximum absorption is at 580 nm.The parent molar absorptivity was 4.66×10 5 L.mol -1 .cm -1 .Beer's law was obeyed within 0～3.0 μg/25 ml selenium.This method has been used to determine trace selenium in blood and urine with good results. |
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| Keywords: | Selenium Rhodamine B iodile spectrophotmetry |
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