| 姜黄素对LPS刺激RAW264.7细胞PGE_2和IL-10影响 |
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| 引用本文： | 陈丽娟,章卓.姜黄素对LPS刺激RAW264.7细胞PGE_2和IL-10影响[J].四川生理科学杂志,2012(2):49-51. |
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| 作者姓名： | 陈丽娟 章卓 |
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| 作者单位： | 泸州医学院附属医院药剂科;泸州医学院药理教研室 |
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| 基金项目： | 泸州医学院青年基金项目(编号:2011017) |
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| 摘 要： | 目的:观察姜黄素对脂多糖刺激小鼠RAW264.7细胞炎症因子的影响。方法:体外培养小鼠RAW264.7细胞,脂多糖刺激小鼠RAW264.7细胞分泌细胞因子,用不同浓度姜黄素进行干预,ELISA法检测培养上清液中抗炎细胞因子IL-10和炎症因子PGE2含量。结果:姜黄素可抑制脂多糖刺激小鼠RAW264.7细胞释放PGE2,促进抗炎因子IL-10表达,并呈一定量效关系,其影响细胞因子释放作用与细胞毒作用无关。结论:姜黄素呈浓度依赖性地抑制脂多糖刺激小鼠RAW264.7细胞释放PGE2,促进IL-10表达。
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| 关 键 词： | 姜黄素 脂多糖 RAW264.7细胞 细胞因子 |
Effects of curcumin on PGE2 and IL-10 released from RAW 264.7 cells induced by LPS |
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| Chen Li-juan,Zhang Zhuo.Effects of curcumin on PGE2 and IL-10 released from RAW 264.7 cells induced by LPS[J].Sichuan Journal of Physiological Sciences,2012(2):49-51. |
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| Authors: | Chen Li-juan Zhang Zhuo |
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| Institution: | 1.Department of Pharmacy,Affiliated Hospital of Luzhou Medical College,Sichuan Luzhou 646000;2Department of Pharmacology,Luzhou Medical College,Sichuan Luzhou 646000) |
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| Abstract: | Objective:To observe the effects of curcumin（Cur） on inflammatory cytokines released from RAW264.7 cells induced by lipopolysaccharides（LPS）.Method: RAW264.7 cell lines were cultivated and released cytokines induced by LPS.IL-10 and PGE2 were detected by enzyme linked immunosolbent assay（ELISA）.Result: Cur could inhibit the content of PGE2 and improve the content of IL-10 in a dose-dependent manner markedly.The effects of Cur on inflammatory cytokines released from RAW264.7 cells had nothing to do with the cytotoxic action.Conclusion: Cur could inhibit PGE2 and increase IL-10 in RAW264.7 cell lines induced by LPS in a dose-dependent manner. |
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| Keywords: | Curcumin Lipopolysaccharides RAW264 7 cell line Cytokines |
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