实时荧光定量PCR与酶联定量PCR技术在 HBV-DNA载量检测中的比较研究

目的:比较实时荧光定量PCR(RQ-PCR)与酶联定量PCR(ELQ-PCR)技术在检测HBV感染血清HBV-DNA载量的灵敏度,精确性.为乙型肝炎临床抗病毒治疗和评价药物疗效提供可靠的依据.方法:应用RQ-PCR方法检测212例(4组)HBV感染者血清,ELQ-PCR方法检测228例(4组)HBV感染者血清.结果:RQ-PCR和ELQ-PCR方法检测HBV-DNA,其阳性检出率分别为HBsAg,HBeAg,HBcAb-IgG( )组100%和82.61%;HBsAg,HBeAb,HBcAb-IgG( )组90.63%和73.21%;HBsAg,HBcIgG,HBcAb-IgM( )组80.00%和57.58%;HBsAg,HB-cAb-IgG( )组70.15%和51.43%.HBV-DNA载量的阳性检出率,经统计学处理有显著性差异(P＜0.05).结论:RQ-CPR方法检测HBV-DNA载量在实时监测,节时快速,高灵敏,高精确度等方面都更优于ELQ-PCR.

Comparative study of RQ-PCR and ELQ-PCR in the detection of HBV-DNA load

Objective: To compare the sensitivity and accuracy of RQ-PCR and ELQ-PCR in detecting the HBV-DNA load and to provide a reliable basis for clinical antiviral therapy and the evaluation of the curative effect of drugs. Methods:Two-hundred and Twelve and 228 serum samples with positive HBV markers were detected by RQ-PCR and ELQ-PCR, respectively. Both the samples detected by RQ-PCR and ELQ-PCR were equally divided into 4 groups: group 1 with HBsAg(+), HBeAg(+), HBcAb-IgG(+), group 2 with HBsAg(+), HBeAb(+), HBcAb-IgG(+), group 3 with HBsAg(+), HBcIgG(+), HBcAb-IgM (+), and group 4 with HBsAg(+), HBcAb-IgG(+). Results:The positive rate of HBV-DNA detected by RQ-PCR and ELQ-PCR in 4 groups were 100.00% and 82.61%, 90.63% and 73.21%, 80.00% and 57.58%, and 70.15% and 51.43%(P<0.05). Conclusion:The sensitivity and accuracy of RQ-PCR are superior to those of ELQ-PCR in detecting the HBV-DNA load.