低密度基因芯片技术检测人乳头瘤病毒基因型

 目的]利用低密度基因芯片方法对人乳头瘤病毒（HPV）进行分型检测,建立一种经济实用的临床HPV感染的基因型检测方法.方法]利用低密度基因芯片方法对145例阴道镜门诊病人的宫颈拭子标本进行HPV-DNA检测并分型.结果]被检人群中共检测出57例HPV阳性,88例HPV阴性,HPV的感染率为39.3%,阳性标本中检测出12种高危型别,2种低危型别.其中单型感染51例（89%）,混合感染6例（11%）,16,31,18,58型检出率较高.结论]低密度基因芯片是一种快速、简便、特异性强、灵敏度高的检测方法,在HPV和宫颈癌的筛查与诊断中有很大的应用价值.

Detecting and Genotyping of Human Papillomavirus by Low-Density DNA Array

Objective] To establish a high efficient and simple method for detecting and genotyping HPV from clinical sample. Methods] Cervical scrape samples were collected from 145 patients who attending the clinic of colposcopy. DNA was extracted and amplified by PCR with general primer set. All PCR-positive samples were then genotyped by low-density DNA array. Results] HPV-DNA was detected in 39.3% of the samples by low-density DNA array. Fifty-one patients were infected with single-type HPV and 6 patients were infected with multiple-type HPV. Twelve high risk types (16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66) and 2 low risk types (6, 11) were detected. The most commonly found high-risk types were HPV-16, HPV-31, HPV-18, and HPV-58. Conclusion] Low-density DNA array is a rapid, convenient method for detecting of HPV DNA with high sensitivity and high specificity in diverse clinical sample. It is a valuable method for the molecular diagnosis and epidemiology of this important virus.